Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

W. S. Grayburn; G. B. Collins; D. F. Hildebrand

doi:10.1038/nbt0692-675

Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

W. S. Grayburn
, G. B. Collins
, D. F. Hildebrand

Plant and Soil Sciences

Producción científica: Article › revisión exhaustiva

39 Citas (Scopus)

Resumen

Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

Idioma original	English
Páginas (desde-hasta)	675-678
Número de páginas	4
Publicación	Bio/Technology
Volumen	10
N.º	6
DOI	https://doi.org/10.1038/nbt0692-675
Estado	Published - jun 1992

ASJC Scopus subject areas

Biotechnology

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abstract = "Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.",

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AB - Nicotiana tabacum tissue was transformed with a rat stearyl-CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found in the phosphatidylcholine fraction of desaturase-transformed leaves, but not in leaves transformed with vector sequences. Plant transformation was verified by polymerase chain reaction (PCR) amplification of total leaf DNA.

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Fatty acid alteration by a Δ9 desaturase in transgenic tobacco tissue

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