Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions

I. Dobrinski; J. R. Jacob; B. C. Tennant; B. A. Ball

doi:10.1016/S0093-691X(99)00179-X

Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions

I. Dobrinski
, J. R. Jacob
, B. C. Tennant
, B. A. Ball

Veterinary Science

Producción científica: Article › revisión exhaustiva

15 Citas (Scopus)

Resumen

Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.

Idioma original	English
Páginas (desde-hasta)	875-885
Número de páginas	11
Publicación	Theriogenology
Volumen	52
N.º	5
DOI	https://doi.org/10.1016/S0093-691X(99)00179-X
Estado	Published - oct 1 1999

Nota bibliográfica

Funding Information:
D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.

Financiación

D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.

Financiadores
Harry M. Zweig Memorial Fund for Equine Research

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

Acceder al documento

10.1016/S0093-691X(99)00179-X

Otros archivos y enlaces

Citar esto

@article{f1a411786fb74aa3a955d89ce227c2f3,

title = "Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions",

abstract = "Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.",

keywords = "Horse, Oviduct, SV40, Sperm binding",

author = "I. Dobrinski and Jacob, \{J. R.\} and Tennant, \{B. C.\} and Ball, \{B. A.\}",

note = "Funding Information: D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.",

year = "1999",

month = oct,

day = "1",

doi = "10.1016/S0093-691X(99)00179-X",

language = "English",

volume = "52",

pages = "875--885",

number = "5",

}

TY - JOUR

T1 - Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions

AU - Dobrinski, I.

AU - Jacob, J. R.

AU - Tennant, B. C.

AU - Ball, B. A.

N1 - Funding Information: D.F. Antczak for the antibodies against equine MHC I and II. This study was supported by the Harry M. Zweig Memorial Fund for Equine Research. I. Dobrinski was a recipient of a Graduate Research Assistantship awarded by the College of Veterinary Medicine, Cornell University. a Correspondence and reprint requests: Dr. I. Dobrinski, Center for Animal Transgenesis and Germ Cell Research, 147 Myrin Bldg., New Bolton Center, University of Pennsylvania, 382 W. Street Rd., Kennett Square, PA 19348.

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.

AB - Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 Tag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.

KW - Horse

KW - Oviduct

KW - SV40

KW - Sperm binding

UR - https://www.scopus.com/pages/publications/0033212934

UR - https://www.scopus.com/pages/publications/0033212934#tab=citedBy

U2 - 10.1016/S0093-691X(99)00179-X

DO - 10.1016/S0093-691X(99)00179-X

M3 - Article

C2 - 10735127

AN - SCOPUS:0033212934

SN - 0093-691X

VL - 52

SP - 875

EP - 885

JO - Theriogenology

JF - Theriogenology

IS - 5

ER -

Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto