Genetically fused single-chain anti-Salmonella antibody with aequorin: A bioluminescence immunoassay for a Salmonella antigen

Jianquan Wang; C. Mark Ensor; Ginette J. Dubuc; Saran A. Narang; Sylvia Daunert

doi:10.1016/S0003-2670(01)00852-2

Genetically fused single-chain anti-Salmonella antibody with aequorin: A bioluminescence immunoassay for a Salmonella antigen

Jianquan Wang
, C. Mark Ensor
, Ginette J. Dubuc
, Saran A. Narang
, Sylvia Daunert

Producción científica: Article › revisión exhaustiva

4 Citas (Scopus)

Resumen

We report the development of a competitive heterogeneous immunoassay for BSA-conjugated Salmonella antigen using a fusion protein between an anti-Salmonella single-chain antibody (scFv) and the photo-protein aequorin (AEQ). It has been demonstrated that the fusion protein (scFvLAEQ) maintains both the antibody binding affinity toward the Salmonella antigen as well as the luminescence properties of the photoprotein. Using scFvLAEQ, the competitive immunoassay gave a detection limit of 10 μgml^-1 of Salmonella antigen. The assay eliminates the need for a labeled secondary antibody usually required in enzyme-linked immuosorbent assays (ELISAs), and therefore, it may find potential applications in detecting Salmonella contamination.

Idioma original	English
Páginas (desde-hasta)	255-263
Número de páginas	9
Publicación	Analytica Chimica Acta
Volumen	435
N.º	2
DOI	https://doi.org/10.1016/S0003-2670(01)00852-2
Estado	Published - may 24 2001

Financiación

This work was supported by grants from the US National Institute of Health (Grant GM 47915) to Sylvia Daunert and Department of Energy (DE FG0595ER62010) to S. Daunert. S. Daunert is a Cottrell Scholar and a Lilly Faculty Awardee. The authors would like to thank Dr. Leonidas G. Bachas for helpful discussions. We are also thankful to Dr. Roger MacKenzie and Tomoko Hirama for the BIAcore analysis.

Financiadores	Número del financiador
National Institutes of Health (NIH)	DE FG0595ER62010, GM 47915

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

Acceder al documento

10.1016/S0003-2670(01)00852-2

Otros archivos y enlaces

Citar esto

@article{7a939f6f74f843e89683032372273935,

title = "Genetically fused single-chain anti-Salmonella antibody with aequorin: A bioluminescence immunoassay for a Salmonella antigen",

abstract = "We report the development of a competitive heterogeneous immunoassay for BSA-conjugated Salmonella antigen using a fusion protein between an anti-Salmonella single-chain antibody (scFv) and the photo-protein aequorin (AEQ). It has been demonstrated that the fusion protein (scFvLAEQ) maintains both the antibody binding affinity toward the Salmonella antigen as well as the luminescence properties of the photoprotein. Using scFvLAEQ, the competitive immunoassay gave a detection limit of 10 μgml-1 of Salmonella antigen. The assay eliminates the need for a labeled secondary antibody usually required in enzyme-linked immuosorbent assays (ELISAs), and therefore, it may find potential applications in detecting Salmonella contamination.",

keywords = "Aequorin, Bioluminescence, Immunoassay, Salmonella detection, Single-chain antibody",

author = "Jianquan Wang and Ensor, \{C. Mark\} and Dubuc, \{Ginette J.\} and Narang, \{Saran A.\} and Sylvia Daunert",

year = "2001",

month = may,

day = "24",

doi = "10.1016/S0003-2670(01)00852-2",

language = "English",

volume = "435",

pages = "255--263",

number = "2",

}

TY - JOUR

T1 - Genetically fused single-chain anti-Salmonella antibody with aequorin

T2 - A bioluminescence immunoassay for a Salmonella antigen

AU - Wang, Jianquan

AU - Ensor, C. Mark

AU - Dubuc, Ginette J.

AU - Narang, Saran A.

AU - Daunert, Sylvia

PY - 2001/5/24

Y1 - 2001/5/24

N2 - We report the development of a competitive heterogeneous immunoassay for BSA-conjugated Salmonella antigen using a fusion protein between an anti-Salmonella single-chain antibody (scFv) and the photo-protein aequorin (AEQ). It has been demonstrated that the fusion protein (scFvLAEQ) maintains both the antibody binding affinity toward the Salmonella antigen as well as the luminescence properties of the photoprotein. Using scFvLAEQ, the competitive immunoassay gave a detection limit of 10 μgml-1 of Salmonella antigen. The assay eliminates the need for a labeled secondary antibody usually required in enzyme-linked immuosorbent assays (ELISAs), and therefore, it may find potential applications in detecting Salmonella contamination.

AB - We report the development of a competitive heterogeneous immunoassay for BSA-conjugated Salmonella antigen using a fusion protein between an anti-Salmonella single-chain antibody (scFv) and the photo-protein aequorin (AEQ). It has been demonstrated that the fusion protein (scFvLAEQ) maintains both the antibody binding affinity toward the Salmonella antigen as well as the luminescence properties of the photoprotein. Using scFvLAEQ, the competitive immunoassay gave a detection limit of 10 μgml-1 of Salmonella antigen. The assay eliminates the need for a labeled secondary antibody usually required in enzyme-linked immuosorbent assays (ELISAs), and therefore, it may find potential applications in detecting Salmonella contamination.

KW - Aequorin

KW - Bioluminescence

KW - Immunoassay

KW - Salmonella detection

KW - Single-chain antibody

UR - https://www.scopus.com/pages/publications/0035942527

UR - https://www.scopus.com/pages/publications/0035942527#tab=citedBy

U2 - 10.1016/S0003-2670(01)00852-2

DO - 10.1016/S0003-2670(01)00852-2

M3 - Article

AN - SCOPUS:0035942527

SN - 0003-2670

VL - 435

SP - 255

EP - 263

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 2

ER -

Genetically fused single-chain anti-Salmonella antibody with aequorin: A bioluminescence immunoassay for a Salmonella antigen

Resumen

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto