Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants

Yu Zhang; Xiangwen Luo; Deyong Zhang; Xian OuYang; Zhanhong Zhang; Fan Li; Chengjia Zhang; Jianxin Chen; Xuguo Zhou; Songbai Zhang; Yong Liu

doi:10.1007/s42161-018-00235-w

Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants

Yu Zhang, Xiangwen Luo, Deyong Zhang, Xian OuYang, Zhanhong Zhang, Fan Li, Chengjia Zhang, Jianxin Chen, Xuguo Zhou, Songbai Zhang, Yong Liu

Entomology

Producción científica: Article › revisión exhaustiva

2 Citas (Scopus)

Resumen

In 2013, a chili pepper (Capsicum annuum cv. ‘Favorite’) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.

Idioma original	English
Páginas (desde-hasta)	559-564
Número de páginas	6
Publicación	Journal of Plant Pathology
Volumen	101
N.º	3
DOI	https://doi.org/10.1007/s42161-018-00235-w
Estado	Published - ago 15 2019

Nota bibliográfica

Publisher Copyright:
© 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.).

Financiación

This work was partially funded by the National Natural Science Foundation of China (31701765), the Agriculture Research System of China (CARS-25-B-05), the Hunan Talent Project (2016RS2019) and the Science and Technology Project of Hunan Academy of Agricultural Science (2017JC43). This work was partially funded by the National Natural Science Foundation of China (31701765), the Agriculture Research System of China (CARS-25-B-05), the Hunan Talent Project (2016RS2019) and the Science and Technology Project of Hunan Academy of Agricultural Science (2017JC43).

Financiadores	Número del financiador
Hunan Talent Project	2016RS2019
Science and Technology Project of Hunan Academy of Agricultural Science	2017JC43
National Natural Science Foundation of China (NSFC)	31701765
National Natural Science Foundation of China (NSFC)
Agriculture Research System of China	CARS-25-B-05
Agriculture Research System of China
Hunan University of Science and Technology

ASJC Scopus subject areas

Plant Science

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10.1007/s42161-018-00235-w

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title = "Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants",

abstract = "In 2013, a chili pepper (Capsicum annuum cv. {\textquoteleft}Favorite{\textquoteright}) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99\% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.",

keywords = "Coat protein gene, Complete genome sequence, Pepper mottle virus, Virus evolution, Virus recombination",

author = "Yu Zhang and Xiangwen Luo and Deyong Zhang and Xian OuYang and Zhanhong Zhang and Fan Li and Chengjia Zhang and Jianxin Chen and Xuguo Zhou and Songbai Zhang and Yong Liu",

note = "Publisher Copyright: {\textcopyright} 2019, Societ{\`a} Italiana di Patologia Vegetale (S.I.Pa.V.).",

year = "2019",

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doi = "10.1007/s42161-018-00235-w",

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T1 - Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants

AU - Zhang, Yu

AU - Luo, Xiangwen

AU - Zhang, Deyong

AU - OuYang, Xian

AU - Zhang, Zhanhong

AU - Li, Fan

AU - Zhang, Chengjia

AU - Chen, Jianxin

AU - Zhou, Xuguo

AU - Zhang, Songbai

AU - Liu, Yong

PY - 2019/8/15

Y1 - 2019/8/15

N2 - In 2013, a chili pepper (Capsicum annuum cv. ‘Favorite’) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.

AB - In 2013, a chili pepper (Capsicum annuum cv. ‘Favorite’) sample displaying crinkled and mottled leaves was collected from a field in the Hunan Province, China. Small RNA (sRNA) sequencing identified the putative virus as pepper mottle virus (PepMoV), which was confirmed by RT-PCR analysis. Full length sequence of this virus strain (designated as HN) was obtained and shown to be typical of a potyvirus. Coat protein (CP) genes of nine PepMoV isolates obtained from south China were sequenced and they shared 89 to 99% sequence identity with PepMoV CP sequences retrieved from GenBank. Phylogenetic analysis, and the level of recombination, gene flow and genetic differention revealed that the PepMoV isolates of Chinese lineage clustered within the south-east group. PepMoV has now been detected in three different provinces of southern part of China.

KW - Coat protein gene

KW - Complete genome sequence

KW - Pepper mottle virus

KW - Virus evolution

KW - Virus recombination

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SN - 1125-4653

VL - 101

SP - 559

EP - 564

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

IS - 3

ER -

Genome and phylogenetic analyses of Chinese pepper mottle virus isolates from chili pepper plants

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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Huella

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