Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati; Liuyin Ma; Arthur G. Hunt

doi:10.1007/978-1-4939-2175-1_14

Genome-wide determination of poly(A) site choice in plants

Pratap Kumar Pati
, Liuyin Ma
, Arthur G. Hunt

Plant and Soil Sciences

Producción científica: Chapter › revisión exhaustiva

1 Cita (Scopus)

Resumen

Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

Idioma original	English
Título de la publicación alojada	Polyadenylation in Plants
Subtítulo de la publicación alojada	Methods and Protocols
Páginas	159-174
Número de páginas	16
ISBN (versión digital)	9781493921751
DOI	https://doi.org/10.1007/978-1-4939-2175-1_14
Estado	Published - dic 8 2014

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Publisher Copyright:
© Springer Science+Business Media New York 2015. All rights are reserved.

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Medicine
General Biochemistry, Genetics and Molecular Biology

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AU - Ma, Liuyin

AU - Hunt, Arthur G.

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Y1 - 2014/12/8

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AB - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.

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Genome-wide determination of poly(A) site choice in plants

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