TY - JOUR
T1 - Genome-wide determination of poly(A) site choice in plants
AU - Pati, Pratap Kumar
AU - Ma, Liuyin
AU - Hunt, Arthur G.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.
AB - Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using highthroughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3′-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.
KW - Alternative polyadenylation
KW - Gene expression
KW - High-throughput sequencing
KW - PAT-seq
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U2 - 10.1007/978-1-4939-2175-1_14
DO - 10.1007/978-1-4939-2175-1_14
M3 - Article
C2 - 25487212
AN - SCOPUS:84916244763
SN - 1064-3745
VL - 1255
SP - 159
EP - 174
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -