TY - CHAP
T1 - Genome-wide screens for identification of host factors in viral replication
AU - Panavas, Tadas
AU - Serviene, Elena
AU - Pogany, Judit
AU - Nagy, Peter D.
PY - 2008
Y1 - 2008
N2 - The central step in virus infection cycle is replication, which depends on viral and host factors. Model hosts, such as yeast, can be very valuable to identify host factors and study the functional interactions of host factors with viral proteins and/or the virus nucleic acids. The advantages of using yeast include the availability of (i) single gene-deletion library, (ii) the essential gene library (yTHC), (iii) the controllable small or large-scale expression of viral proteins and nucleic acids, and (iv) the rapid growth of yeast strains. Here, we describe procedures, which facilitate high-throughput analysis of tombusvirus replication in yeast.
AB - The central step in virus infection cycle is replication, which depends on viral and host factors. Model hosts, such as yeast, can be very valuable to identify host factors and study the functional interactions of host factors with viral proteins and/or the virus nucleic acids. The advantages of using yeast include the availability of (i) single gene-deletion library, (ii) the essential gene library (yTHC), (iii) the controllable small or large-scale expression of viral proteins and nucleic acids, and (iv) the rapid growth of yeast strains. Here, we describe procedures, which facilitate high-throughput analysis of tombusvirus replication in yeast.
KW - High-throughput transformation
KW - High-throughput viral RNA extraction
KW - Host factors
KW - Northern blotting
KW - Tombusviruses
KW - Yeast
UR - https://www.scopus.com/pages/publications/84934438509
UR - https://www.scopus.com/pages/publications/84934438509#tab=citedBy
U2 - 10.1007/978-1-59745-102-4_41
DO - 10.1007/978-1-59745-102-4_41
M3 - Chapter
C2 - 18370284
AN - SCOPUS:84934438509
SN - 9781588298270
T3 - Methods in Molecular Biology
SP - 615
EP - 624
BT - Plant Virology Protocols
ER -