High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy

Nicolas Grillet

doi:10.1016/j.xpro.2022.101213

High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy

Nicolas Grillet

Producción científica: Article › revisión exhaustiva

6 Citas (Scopus)

Resumen

Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).

Idioma original	English
Número de artículo	101213
Publicación	STAR Protocols
Volumen	3
N.º	1
DOI	https://doi.org/10.1016/j.xpro.2022.101213
Estado	Published - mar 18 2022

Nota bibliográfica

Publisher Copyright:
© 2022 The Author(s)

Financiación

License for the graphics of the ear (item ID: 2051711780) and SEM microscope (item ID: 753979801) was purchased from Shutterstock.com . The work was funded by the Department of Otolaryngology-Head and Neck Surgery startup funding and National Institute on Deafness, and Other Communication Disorders Grants 1R21DC019457-01 and RO1-DC-016409-01A1 . We thank the Stanford Nano Shared Facilities Richard Chin, Juliet Jamtgaard, and Tobi Beetz for technical help with the SEM microscope. The National Science Foundation Award ECCS-2026822 supports the Stanford Nano Shared Facilities. We thank the Grillet lab members and in particular Katharine Miller for feedback on the manuscript. Finally, we thank Shari and Kenneth Eberts, the Oberndorf family, and the SICHL contributors for their support.

Financiadores	Número del financiador
National Science Foundation Arctic Social Science Program	ECCS-2026822
National Institute on Deafness and Other Communication Disorders	R21DC019457, R01DC016409
National Institute on Deafness and Other Communication	1R21DC019457-01, RO1-DC-016409-01A1

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

Acceder al documento

10.1016/j.xpro.2022.101213

Otros archivos y enlaces

Citar esto

@article{908f2dc9732c4e01b1ee6af8e4b1922c,

title = "High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy",

abstract = "Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).",

keywords = "Cell Biology, Microscopy, Neuroscience",

author = "Nicolas Grillet",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = mar,

day = "18",

doi = "10.1016/j.xpro.2022.101213",

language = "English",

volume = "3",

number = "1",

}

TY - JOUR

T1 - High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy

AU - Grillet, Nicolas

PY - 2022/3/18

Y1 - 2022/3/18

N2 - Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).

AB - Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).

KW - Cell Biology

KW - Microscopy

KW - Neuroscience

UR - https://www.scopus.com/pages/publications/85125531219

UR - https://www.scopus.com/pages/publications/85125531219#tab=citedBy

U2 - 10.1016/j.xpro.2022.101213

DO - 10.1016/j.xpro.2022.101213

M3 - Article

C2 - 35257116

AN - SCOPUS:85125531219

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 101213

ER -

High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto