Identification and characterization of two distinctive RNA binding activities in pea nuclear extracts

Jaydip Das Gupta; Qingshun Li; Arthur G. Hunt

doi:10.1007/BF03263025

Identification and characterization of two distinctive RNA binding activities in pea nuclear extracts

Jaydip Das Gupta
, Qingshun Li
, Arthur G. Hunt

Plant and Soil Sciences

Producción científica: Article › revisión exhaustiva

1 Cita (Scopus)

Resumen

We have identified and purified two RNA binding factors from pea nuclear extracts. One factor (RBF-1) consisted of at least two polypeptides with molecular weights of approximately 27 and 59 kD; each of these polypeptides could be crosslinked to labelled RNA by ultraviolet light. The other factor (RBF-2) also consisted of two polypeptides (of approximately 93 and 126 kD in size) that could be crosslinked to RNA by UV. Both factors showed general preferences for single stranded RNA. However, RBF-1 displayed a preference for poly(A) and poly(U) over poly(G) or poly(C), while RBF-2 had a strong preference for poly(U) over the other three homopolymers. These properties are suggestive of possible roles for these factors in RNA metabolism in plant nuclei.

Idioma original	English
Páginas (desde-hasta)	1-5
Número de páginas	5
Publicación	Journal of Plant Biochemistry and Biotechnology
Volumen	7
N.º	1
DOI	https://doi.org/10.1007/BF03263025
Estado	Published - ene 1998

Nota bibliográfica

Funding Information:
I am greatly indebted to Dr. E. Orias for many constructive discussions on the model of copy number control for macronuclear fragments and to a number of colleagues who assisted with constructive criticism of this manuscript and who communicated the results of their investigations prior to their publication. In particular, 1 wish to thank Drs. S. Allen, E. Blackburn, K. Conover, K. Jones, V. Merriam, E. Orias, R. Pearlman. and M.-C. Yao. Experimental work in the author’s laboratory was supported by research grants from the National Science Foundation (PCM-8215666) and the Academic Senate of the University of California (2455).

Financiación

I am greatly indebted to Dr. E. Orias for many constructive discussions on the model of copy number control for macronuclear fragments and to a number of colleagues who assisted with constructive criticism of this manuscript and who communicated the results of their investigations prior to their publication. In particular, 1 wish to thank Drs. S. Allen, E. Blackburn, K. Conover, K. Jones, V. Merriam, E. Orias, R. Pearlman. and M.-C. Yao. Experimental work in the author’s laboratory was supported by research grants from the National Science Foundation (PCM-8215666) and the Academic Senate of the University of California (2455).

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Plant Science

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@article{757d7fefaf3b44fd9087434463b07189,

title = "Identification and characterization of two distinctive RNA binding activities in pea nuclear extracts",

abstract = "We have identified and purified two RNA binding factors from pea nuclear extracts. One factor (RBF-1) consisted of at least two polypeptides with molecular weights of approximately 27 and 59 kD; each of these polypeptides could be crosslinked to labelled RNA by ultraviolet light. The other factor (RBF-2) also consisted of two polypeptides (of approximately 93 and 126 kD in size) that could be crosslinked to RNA by UV. Both factors showed general preferences for single stranded RNA. However, RBF-1 displayed a preference for poly(A) and poly(U) over poly(G) or poly(C), while RBF-2 had a strong preference for poly(U) over the other three homopolymers. These properties are suggestive of possible roles for these factors in RNA metabolism in plant nuclei.",

keywords = "Binding activities, Nuclear extracts, Pea, RNA",

author = "\{Das Gupta\}, Jaydip and Qingshun Li and Hunt, \{Arthur G.\}",

note = "Funding Information: I am greatly indebted to Dr. E. Orias for many constructive discussions on the model of copy number control for macronuclear fragments and to a number of colleagues who assisted with constructive criticism of this manuscript and who communicated the results of their investigations prior to their publication. In particular, 1 wish to thank Drs. S. Allen, E. Blackburn, K. Conover, K. Jones, V. Merriam, E. Orias, R. Pearlman. and M.-C. Yao. Experimental work in the author{\textquoteright}s laboratory was supported by research grants from the National Science Foundation (PCM-8215666) and the Academic Senate of the University of California (2455).",

year = "1998",

month = jan,

doi = "10.1007/BF03263025",

language = "English",

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AU - Das Gupta, Jaydip

AU - Li, Qingshun

AU - Hunt, Arthur G.

N1 - Funding Information: I am greatly indebted to Dr. E. Orias for many constructive discussions on the model of copy number control for macronuclear fragments and to a number of colleagues who assisted with constructive criticism of this manuscript and who communicated the results of their investigations prior to their publication. In particular, 1 wish to thank Drs. S. Allen, E. Blackburn, K. Conover, K. Jones, V. Merriam, E. Orias, R. Pearlman. and M.-C. Yao. Experimental work in the author’s laboratory was supported by research grants from the National Science Foundation (PCM-8215666) and the Academic Senate of the University of California (2455).

PY - 1998/1

Y1 - 1998/1

N2 - We have identified and purified two RNA binding factors from pea nuclear extracts. One factor (RBF-1) consisted of at least two polypeptides with molecular weights of approximately 27 and 59 kD; each of these polypeptides could be crosslinked to labelled RNA by ultraviolet light. The other factor (RBF-2) also consisted of two polypeptides (of approximately 93 and 126 kD in size) that could be crosslinked to RNA by UV. Both factors showed general preferences for single stranded RNA. However, RBF-1 displayed a preference for poly(A) and poly(U) over poly(G) or poly(C), while RBF-2 had a strong preference for poly(U) over the other three homopolymers. These properties are suggestive of possible roles for these factors in RNA metabolism in plant nuclei.

AB - We have identified and purified two RNA binding factors from pea nuclear extracts. One factor (RBF-1) consisted of at least two polypeptides with molecular weights of approximately 27 and 59 kD; each of these polypeptides could be crosslinked to labelled RNA by ultraviolet light. The other factor (RBF-2) also consisted of two polypeptides (of approximately 93 and 126 kD in size) that could be crosslinked to RNA by UV. Both factors showed general preferences for single stranded RNA. However, RBF-1 displayed a preference for poly(A) and poly(U) over poly(G) or poly(C), while RBF-2 had a strong preference for poly(U) over the other three homopolymers. These properties are suggestive of possible roles for these factors in RNA metabolism in plant nuclei.

KW - Binding activities

KW - Nuclear extracts

KW - Pea

KW - RNA

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M3 - Article

AN - SCOPUS:13144252164

SN - 0971-7811

VL - 7

SP - 1

EP - 5

JO - Journal of Plant Biochemistry and Biotechnology

JF - Journal of Plant Biochemistry and Biotechnology

IS - 1

ER -

Identification and characterization of two distinctive RNA binding activities in pea nuclear extracts

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Nota bibliográfica

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