Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Yueh Fen Li; Jian Shi; Michael C. Nelson; Po Hsu Chen; Joerg Graf; Yebo Li; Zhongtang Yu

doi:10.1016/j.biortech.2015.10.078

Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Yueh Fen Li
, Jian Shi
, Michael C. Nelson
, Po Hsu Chen
, Joerg Graf
, Yebo Li
, Zhongtang Yu

Producción científica: Article › revisión exhaustiva

53 Citas (Scopus)

Resumen

The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

Idioma original	English
Páginas (desde-hasta)	744-752
Número de páginas	9
Publicación	Bioresource Technology
Volumen	200
DOI	https://doi.org/10.1016/j.biortech.2015.10.078
Estado	Published - ene 1 2016

Nota bibliográfica

Publisher Copyright:
© 2015 Elsevier Ltd.

Financiación

This study was partially supported by a Department of Energy (DOE) award ( DE-FG36-05GO85010 ) and a United States Department of Agriculture/ National Institute of Food and Agriculture (USDA/NIFA) award ( 2012-10008-20302 ).

Financiadores	Número del financiador
U.S. Department of Energy EPSCoR	DE-FG36-05GO85010
U.S. Department of Energy EPSCoR
U.S. Department of Agriculture
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative	2012-10008-20302
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Affordable and clean energy

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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title = "Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover",

abstract = "The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.",

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AU - Li, Yueh Fen

AU - Shi, Jian

AU - Nelson, Michael C.

AU - Chen, Po Hsu

AU - Graf, Joerg

AU - Li, Yebo

AU - Yu, Zhongtang

PY - 2016/1/1

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AB - The objective of this study was to understand how the non-microbial factors of L-AD effluent affected the microbiome composition and successions in the SS-AD digesters using both Illumina sequencing and qPCR quantification of major genera of methanogens. The SS-AD digesters started with a feedstock/total effluent (F/Et) ratio 2.2 (half of the effluent was autoclaved) performed stably, while the SS-AD digesters started with a 4.4 F/Et ratio (no autoclaved effluent) suffered from digester acidification, accumulation of volatile fatty acids, and ceased biogas production two weeks after startup. Some bacteria and methanogens were affected by non-microbial factors of the L-AD fluent. Alkalinity, the main difference between the two F/Et ratios, may be the crucial factor when SS-AD digesters were started using L-AD effluent.

KW - 16S rRNA gene

KW - Illumina sequencing

KW - Metagenomics

KW - Microbiome

KW - Succession

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JO - Bioresource Technology

JF - Bioresource Technology

ER -

Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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Huella

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