Improving human skeletal muscle myosin heavy chain fiber typing efficiency

Kevin A. Murach; James R. Bagley; Kathryn A. McLeland; Jose A. Arevalo; Anthony B. Ciccone; Kylie K. Malyszek; Yuan Wen; Andrew J. Galpin

doi:10.1007/s10974-016-9441-9

Improving human skeletal muscle myosin heavy chain fiber typing efficiency

Kevin A. Murach
, James R. Bagley
, Kathryn A. McLeland
, Jose A. Arevalo
, Anthony B. Ciccone
, Kylie K. Malyszek
, Yuan Wen
, Andrew J. Galpin

Producción científica: Article › revisión exhaustiva

19 Citas (Scopus)

Resumen

Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.

Idioma original	English
Páginas (desde-hasta)	1-5
Número de páginas	5
Publicación	Journal of Muscle Research and Cell Motility
Volumen	37
N.º	1-2
DOI	https://doi.org/10.1007/s10974-016-9441-9
Estado	Published - abr 1 2016

Nota bibliográfica

Publisher Copyright:
© 2016, Springer International Publishing Switzerland.

Financiación

Thanks to Drs. Scott Trappe, Todd Trappe, Ulrika Raue, and Holmes Finch for their thoughts and feedback on the topic. This work was funded by a California State University, Fullerton Junior Faculty Grant (CSUF03569) to A. J. Galpin and publication costs were defrayed by the Department of Kinesiology, College of Health and Social Sciences, San Francisco State University.

Financiadores	Número del financiador
California State University	CSUF03569

ASJC Scopus subject areas

Physiology
Biochemistry
Cell Biology

Acceder al documento

10.1007/s10974-016-9441-9

Otros archivos y enlaces

Citar esto

@article{5e991b53edfc437aa3149471376826b9,

title = "Improving human skeletal muscle myosin heavy chain fiber typing efficiency",

abstract = "Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.",

keywords = "Hybrid fibers, Muscle biopsy, SDS-PAGE, Vastus lateralis",

author = "Murach, \{Kevin A.\} and Bagley, \{James R.\} and McLeland, \{Kathryn A.\} and Arevalo, \{Jose A.\} and Ciccone, \{Anthony B.\} and Malyszek, \{Kylie K.\} and Yuan Wen and Galpin, \{Andrew J.\}",

note = "Publisher Copyright: {\textcopyright} 2016, Springer International Publishing Switzerland.",

year = "2016",

month = apr,

day = "1",

doi = "10.1007/s10974-016-9441-9",

language = "English",

volume = "37",

pages = "1--5",

number = "1-2",

}

TY - JOUR

T1 - Improving human skeletal muscle myosin heavy chain fiber typing efficiency

AU - Murach, Kevin A.

AU - Bagley, James R.

AU - McLeland, Kathryn A.

AU - Arevalo, Jose A.

AU - Ciccone, Anthony B.

AU - Malyszek, Kylie K.

AU - Wen, Yuan

AU - Galpin, Andrew J.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.

AB - Single muscle fiber sodium dodecyl sulfate polyacrylamide gel-electrophoresis (SDS-PAGE) is a sensitive technique for determining skeletal muscle myosin heavy chain (MHC) composition of human biopsy samples. However, the number of fibers suitable to represent fiber type distribution via this method is undefined. Muscle biopsies were obtained from the vastus lateralis (VL) of nine resistance-trained males (25 ± 1 year, height = 179 ± 5 cm, mass = 82 ± 8 kg). Single fiber MHC composition was determined via SDS-PAGE. VL fiber type distribution [percent MHC I, I/IIa, IIa, IIa/IIx, and total “hybrids” (i.e. I/IIa + IIa/IIx)] was evaluated according to number of fibers analyzed per person (25 vs. 125). VL fiber type distribution did not differ according to number of fibers analyzed (P > 0.05). VL biopsy fiber type distribution of nine subjects is represented by analyzing 25 fibers per person. These data may help minimize cost, personnel-time, and materials associated with this technique, thereby improving fiber typing efficiency in humans.

KW - Hybrid fibers

KW - Muscle biopsy

KW - SDS-PAGE

KW - Vastus lateralis

UR - https://www.scopus.com/pages/publications/84957555829

UR - https://www.scopus.com/pages/publications/84957555829#tab=citedBy

U2 - 10.1007/s10974-016-9441-9

DO - 10.1007/s10974-016-9441-9

M3 - Article

C2 - 26842420

AN - SCOPUS:84957555829

SN - 0142-4319

VL - 37

SP - 1

EP - 5

JO - Journal of Muscle Research and Cell Motility

JF - Journal of Muscle Research and Cell Motility

IS - 1-2

ER -

Improving human skeletal muscle myosin heavy chain fiber typing efficiency

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto