In vitro micromechanical behavior of viable neonatal mouse tibia

A preliminary study of the response of viable neonatal mouse tibia tissue to microscale cyclic and near instantaneous mechanical stimulation was conducted, so as to develop a system for studying the relationship between mechanical stimulation and growth factors. Results show that the storage modulus increases with increasing excitation frequency. Labels of tibia imply that both loaded and unloaded tibiae were viable after one hour of testing. One important conclusion is that the established techniques may provide a unique way of establishing the correlations between the hysteresis loss energy and loss modulus, and the tangent modulus and storage modulus.