Incorporation of ¹⁴C 18:2 into Different Lipid fractions of Glycine max Cotyledons

A pulse-chase study of radiolabled ¹⁴C linoleoyl-CoA was carried out at an early and late stage of embryo triglyceride (TG) synthesis of in order to investigate factors that contribute to fatty acids content of normal oilseeds such as Glycine max (Soybean). Data indicate that ¹⁴C-18:2 is rapidly removed from PC and incorporated directly into TG. Also no significant changes occurred in ¹⁴C -18:3 levels in soybean. This indicates that desaturase activity decreased during the late stage in soybean embryos and most of ¹⁴C -18:2 was released from PC and incorporated into TG without being converted into 18:3.