Induced morphological changes in human small cell lung carcinoma cells

R. C. McGarry; V. Feyles; A. Tuff; J. Chapman; L. M. Jerry

doi:10.1016/0304-3835(91)90078-V

Induced morphological changes in human small cell lung carcinoma cells

R. C. McGarry
, V. Feyles
, A. Tuff
, J. Chapman
, L. M. Jerry

Producción científica: Article › revisión exhaustiva

3 Citas (Scopus)

Resumen

Several theories suggest that lung carcinomas are not totally separate entities, but are derived from a common precursor, probably of endodermal origin [1,2]. The histological classification of lung cancers is complex, with much overlap between groups broadly designated as small cell (SCLC), squamous cell, adenocarcinoma and all others simply termed non-small cell. It is shown here that in vitro exposure of classic, non-adherent SCLC lines to 10 μM 5′ bromodeoxyuridine (BrdU) results in a rapid cell-line dependent change to a morphology consistent with an adherent, non-small cell phenotype. Accompanying this morphological shift is a decreased expression of the amplified N-myc protooncogene. These induced changes underline the morphological relatedness of lung carcinoma cell lines.

Idioma original	English
Páginas (desde-hasta)	67-74
Número de páginas	8
Publicación	Cancer Letters
Volumen	61
N.º	1
DOI	https://doi.org/10.1016/0304-3835(91)90078-V
Estado	Published - dic 9 1991

Financiación

We would like to thank Mary Ilkiw and Laurie Bryant for assistance with the flow cytometry. Supported by the National Cancer Institute of Canada by grants to R. McGarry and L.M. Jerry. V. Feyles is a fellow of the Alberta Cancer Board.

Financiadores
National Cancer Institute of Canada

ODS de las Naciones Unidas

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ASJC Scopus subject areas

Oncology
Cancer Research

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AU - McGarry, R. C.

AU - Feyles, V.

AU - Tuff, A.

AU - Chapman, J.

AU - Jerry, L. M.

PY - 1991/12/9

Y1 - 1991/12/9

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AB - Several theories suggest that lung carcinomas are not totally separate entities, but are derived from a common precursor, probably of endodermal origin [1,2]. The histological classification of lung cancers is complex, with much overlap between groups broadly designated as small cell (SCLC), squamous cell, adenocarcinoma and all others simply termed non-small cell. It is shown here that in vitro exposure of classic, non-adherent SCLC lines to 10 μM 5′ bromodeoxyuridine (BrdU) results in a rapid cell-line dependent change to a morphology consistent with an adherent, non-small cell phenotype. Accompanying this morphological shift is a decreased expression of the amplified N-myc protooncogene. These induced changes underline the morphological relatedness of lung carcinoma cell lines.

KW - 5′-bromodeoxyuridine

KW - differentiation

KW - lung carcinomna

KW - N-myc

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Induced morphological changes in human small cell lung carcinoma cells

Resumen

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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