Influence of serotonin on the kinetics of vesicular release

R. Chase Southard; J. Haggard; M. E. Crider; S. W. Whiteheart; R. L. Cooper

doi:10.1016/S0006-8993(00)02347-7

Influence of serotonin on the kinetics of vesicular release

R. Chase Southard
, J. Haggard
, M. E. Crider
, S. W. Whiteheart
, R. L. Cooper

Producción científica: Article › revisión exhaustiva

37 Citas (Scopus)

Resumen

The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.

Idioma original	English
Páginas (desde-hasta)	16-28
Número de páginas	13
Publicación	Brain Research
Volumen	871
N.º	1
DOI	https://doi.org/10.1016/S0006-8993(00)02347-7
Estado	Published - jul 14 2000

Nota bibliográfica

Funding Information:
Funding was provided by the University of Kentucky Research and Graduate Studies Office (RLC), NSF grant IBN-9808631 (RLC), NSF-ILI-DUE 9850907 (RLC) as well as an undergraduate training fellowship from HHMI (RCS & JH) and NIH grant HL56652 (SWW).

Financiación

Funding was provided by the University of Kentucky Research and Graduate Studies Office (RLC), NSF grant IBN-9808631 (RLC), NSF-ILI-DUE 9850907 (RLC) as well as an undergraduate training fellowship from HHMI (RCS & JH) and NIH grant HL56652 (SWW).

Financiadores	Número del financiador
NSF-ILI-DUE	9850907
RLC
University of Kentucky Research and Graduate Studies Office
National Science Foundation Arctic Social Science Program	IBN-9808631
National Institutes of Health (NIH)
Howard Hughes Medical Institute
National Heart, Lung, and Blood Institute (NHLBI)	R01HL056652

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Influence of serotonin on the kinetics of vesicular release",

abstract = "The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.",

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note = "Funding Information: Funding was provided by the University of Kentucky Research and Graduate Studies Office (RLC), NSF grant IBN-9808631 (RLC), NSF-ILI-DUE 9850907 (RLC) as well as an undergraduate training fellowship from HHMI (RCS \& JH) and NIH grant HL56652 (SWW). ",

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N2 - The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.

AB - The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.

KW - Crayfish

KW - Neuromuscular junction

KW - Neurotransmission

KW - Serotonin

KW - Synapse

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AN - SCOPUS:0034648067

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Influence of serotonin on the kinetics of vesicular release

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ASJC Scopus subject areas

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