Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Nitai C. Hait; Katsuhide Fujita; Robert L. Lester; Robert C. Dickson

doi:10.1016/S0014-5793(02)03636-0

Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Nitai C. Hait
, Katsuhide Fujita
, Robert L. Lester
, Robert C. Dickson

Molecular and Cellular Biochemistry

Producción científica: Article › revisión exhaustiva

20 Citas (Scopus)

Resumen

Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

Idioma original	English
Páginas (desde-hasta)	97-102
Número de páginas	6
Publicación	FEBS Letters
Volumen	532
N.º	1-2
DOI	https://doi.org/10.1016/S0014-5793(02)03636-0
Estado	Published - dic 4 2002

Nota bibliográfica

Funding Information:
This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.

Financiación

This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.

Financiadores	Número del financiador
National Institute of General Medical Sciences	R01GM058507
U.S. Public Health Service

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes",

abstract = "Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.",

keywords = "Phytosphingosine, Sphingolipid, Sphingosine kinase, Sphingosine-1-phosphate",

author = "Hait, \{Nitai C.\} and Katsuhide Fujita and Lester, \{Robert L.\} and Dickson, \{Robert C.\}",

note = "Funding Information: This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.",

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doi = "10.1016/S0014-5793(02)03636-0",

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T1 - Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

AU - Hait, Nitai C.

AU - Fujita, Katsuhide

AU - Lester, Robert L.

AU - Dickson, Robert C.

N1 - Funding Information: This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.

PY - 2002/12/4

Y1 - 2002/12/4

N2 - Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

AB - Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

KW - Phytosphingosine

KW - Sphingolipid

KW - Sphingosine kinase

KW - Sphingosine-1-phosphate

UR - https://www.scopus.com/pages/publications/0037021420

UR - https://www.scopus.com/pages/publications/0037021420#tab=citedBy

U2 - 10.1016/S0014-5793(02)03636-0

DO - 10.1016/S0014-5793(02)03636-0

M3 - Article

C2 - 12459470

AN - SCOPUS:0037021420

SN - 0014-5793

VL - 532

SP - 97

EP - 102

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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