Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers

Bernhard Hennig; Cynthia Enoch; Ching K. Chow

doi:10.1016/0003-9861(86)90431-5

Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers

Bernhard Hennig
, Cynthia Enoch
, Ching K. Chow

Producción científica: Article › revisión exhaustiva

44 Citas (Scopus)

Resumen

Exposure to albumin-bound linoleic acid (60 to 150 μM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (18:2) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. A near maximum albumin transfer was observed after a 2-h incubation with 90 μM 18:2-OOH. Cells exposed to 18:2-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 18:2 at concentrations as high as 150 μM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 18:2, and in particular to 18:2-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.

Idioma original	English
Páginas (desde-hasta)	353-357
Número de páginas	5
Publicación	Archives of Biochemistry and Biophysics
Volumen	248
N.º	1
DOI	https://doi.org/10.1016/0003-9861(86)90431-5
Estado	Published - jul 1986

Nota bibliográfica

Funding Information:
i Supported in part by grant HL 34423 from the National Heart, Lung, and Blood Institute, National Institutes of Health, a grant from the American Heart Association, Kentucky Affiliate; a University of Kentucky Biological Research Support grant; and the Kentucky Agricultural Experiment Station. ‘To whom correspondence should be addressed.

Financiación

i Supported in part by grant HL 34423 from the National Heart, Lung, and Blood Institute, National Institutes of Health, a grant from the American Heart Association, Kentucky Affiliate; a University of Kentucky Biological Research Support grant; and the Kentucky Agricultural Experiment Station. ‘To whom correspondence should be addressed.

Financiadores	Número del financiador
Kentucky Affiliate
National Institutes of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)	R23HL034423
American Heart Association
University of Kentucky
Kentucky Agricultural Experiment Station

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(86)90431-5

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title = "Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers",

abstract = "Exposure to albumin-bound linoleic acid (60 to 150 μM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (18:2) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. A near maximum albumin transfer was observed after a 2-h incubation with 90 μM 18:2-OOH. Cells exposed to 18:2-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 18:2 at concentrations as high as 150 μM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 18:2, and in particular to 18:2-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.",

author = "Bernhard Hennig and Cynthia Enoch and Chow, \{Ching K.\}",

note = "Funding Information: i Supported in part by grant HL 34423 from the National Heart, Lung, and Blood Institute, National Institutes of Health, a grant from the American Heart Association, Kentucky Affiliate; a University of Kentucky Biological Research Support grant; and the Kentucky Agricultural Experiment Station. {\textquoteleft}To whom correspondence should be addressed.",

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T1 - Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers

AU - Hennig, Bernhard

AU - Enoch, Cynthia

AU - Chow, Ching K.

N1 - Funding Information: i Supported in part by grant HL 34423 from the National Heart, Lung, and Blood Institute, National Institutes of Health, a grant from the American Heart Association, Kentucky Affiliate; a University of Kentucky Biological Research Support grant; and the Kentucky Agricultural Experiment Station. ‘To whom correspondence should be addressed.

PY - 1986/7

Y1 - 1986/7

N2 - Exposure to albumin-bound linoleic acid (60 to 150 μM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (18:2) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. A near maximum albumin transfer was observed after a 2-h incubation with 90 μM 18:2-OOH. Cells exposed to 18:2-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 18:2 at concentrations as high as 150 μM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 18:2, and in particular to 18:2-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.

AB - Exposure to albumin-bound linoleic acid (60 to 150 μM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (18:2) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. A near maximum albumin transfer was observed after a 2-h incubation with 90 μM 18:2-OOH. Cells exposed to 18:2-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 18:2 at concentrations as high as 150 μM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 18:2, and in particular to 18:2-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.

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Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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Huella

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