Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse; D. Allan Butterfield

doi:10.1080/00032718808055501

Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse, D. Allan Butterfield

Producción científica: Article › revisión exhaustiva

12 Citas (Scopus)

Resumen

A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

Idioma original	English
Páginas (desde-hasta)	1131-1140
Número de páginas	10
Publicación	Analytical Letters
Volumen	21
N.º	7
DOI	https://doi.org/10.1080/00032718808055501
Estado	Published - ene 1988

Nota bibliográfica

Funding Information:
We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

Financiación

We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

Financiadores	Número del financiador
Tobacco and Health Research Institute
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	RII-86-10671
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032718808055501

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title = "Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16",

abstract = "A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.",

keywords = "CAT-16, Spin-labeling, cationic spin label, erythrocyte membranes, lipid bilayer, lipid-protein interactions, membrane glycoconjugate",

author = "Wyse, \{Joseph W.\} and \{Allan Butterfield\}, D.",

note = "Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.",

year = "1988",

month = jan,

doi = "10.1080/00032718808055501",

language = "English",

volume = "21",

pages = "1131--1140",

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TY - JOUR

T1 - Lipid Specific Spin-Labeling of Erythrocyte Membranes

T2 - Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

AU - Wyse, Joseph W.

AU - Allan Butterfield, D.

N1 - Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

PY - 1988/1

Y1 - 1988/1

N2 - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

AB - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

KW - CAT-16

KW - Spin-labeling

KW - cationic spin label

KW - erythrocyte membranes

KW - lipid bilayer

KW - lipid-protein interactions

KW - membrane glycoconjugate

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U2 - 10.1080/00032718808055501

DO - 10.1080/00032718808055501

M3 - Article

AN - SCOPUS:0023732912

SN - 0003-2719

VL - 21

SP - 1131

EP - 1140

JO - Analytical Letters

JF - Analytical Letters

IS - 7

ER -

Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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