Localization of prostaglandin endoperoxide synthase in the human corpus luteum

S. W. Kauma; T. E. Curry; D. E. Powell; M. R. Clark

doi:10.1093/oxfordjournals.humrep.a137186

Localization of prostaglandin endoperoxide synthase in the human corpus luteum

S. W. Kauma
, T. E. Curry
, D. E. Powell
, M. R. Clark

Producción científica: Article › revisión exhaustiva

5 Citas (Scopus)

Resumen

Prostaglandins have been implicated in both maintenance and luteolysis of the primate corpus luteum. Central to the production of prostaglandins is the enzyme prostaglandin endoperoxide synthase (PGHS). In the present study, we identified the cell types which contain PGHS in 44 human corpora lutea, using immunoperoxidase staining techniques. Intense granular staining was present in the cytoplasm of granulosa lutein cells of tissues obtained from the mid-luteal phase. Theca lutein cells demonstrated a diffuse cytoplasmic staining which was less intense than that observed in granulosa lutein cells. Staining appeared less intense in tisues from the early or late phase. Ovarian stromal cells demonstrated little or no PGHS immunoreactivity. PGHS staining in the corpus luteum of pregnancy was similar in intensity and cell distribution to that of mid-luted corpus luteum. In summary, human corpus luteum contains immunoreactive PGHS which localized mainly to welldifferentiated granulosa lutein cells.

Idioma original	English
Páginas (desde-hasta)	800-804
Número de páginas	5
Publicación	Human Reproduction
Volumen	5
N.º	7
DOI	https://doi.org/10.1093/oxfordjournals.humrep.a137186
Estado	Published - oct 1990

Nota bibliográfica

Funding Information:
Tissue specimens were obtained with the assistance of F.B.Askin, M.D., Department of Pathology, University of North Carolina at Chapel Hill. The technical assistance of Carole Bryant in preparing the luteal sections is gratefully appreciated. The research was supported by NIH grants HD-22299, HD-18968, and the Department of Obstetrics and Gynecology.

Financiación

Tissue specimens were obtained with the assistance of F.B.Askin, M.D., Department of Pathology, University of North Carolina at Chapel Hill. The technical assistance of Carole Bryant in preparing the luteal sections is gratefully appreciated. The research was supported by NIH grants HD-22299, HD-18968, and the Department of Obstetrics and Gynecology.

Financiadores	Número del financiador
Department of Obstetrics and Gynecology
National Institutes of Health (NIH)	HD-22299
Eunice Kennedy Shriver National Institute of Child Health and Human Development	P30HD018968

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1093/oxfordjournals.humrep.a137186

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@article{1ed4c549342d474db00ad2769f6526fc,

title = "Localization of prostaglandin endoperoxide synthase in the human corpus luteum",

abstract = "Prostaglandins have been implicated in both maintenance and luteolysis of the primate corpus luteum. Central to the production of prostaglandins is the enzyme prostaglandin endoperoxide synthase (PGHS). In the present study, we identified the cell types which contain PGHS in 44 human corpora lutea, using immunoperoxidase staining techniques. Intense granular staining was present in the cytoplasm of granulosa lutein cells of tissues obtained from the mid-luteal phase. Theca lutein cells demonstrated a diffuse cytoplasmic staining which was less intense than that observed in granulosa lutein cells. Staining appeared less intense in tisues from the early or late phase. Ovarian stromal cells demonstrated little or no PGHS immunoreactivity. PGHS staining in the corpus luteum of pregnancy was similar in intensity and cell distribution to that of mid-luted corpus luteum. In summary, human corpus luteum contains immunoreactive PGHS which localized mainly to welldifferentiated granulosa lutein cells.",

keywords = "Corpus luteum, Cyclooxygenase, Immunohistochemistry, Prostaglandin endoperoxide synthase",

author = "Kauma, \{S. W.\} and Curry, \{T. E.\} and Powell, \{D. E.\} and Clark, \{M. R.\}",

note = "Funding Information: Tissue specimens were obtained with the assistance of F.B.Askin, M.D., Department of Pathology, University of North Carolina at Chapel Hill. The technical assistance of Carole Bryant in preparing the luteal sections is gratefully appreciated. The research was supported by NIH grants HD-22299, HD-18968, and the Department of Obstetrics and Gynecology.",

year = "1990",

month = oct,

doi = "10.1093/oxfordjournals.humrep.a137186",

language = "English",

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T1 - Localization of prostaglandin endoperoxide synthase in the human corpus luteum

AU - Kauma, S. W.

AU - Curry, T. E.

AU - Powell, D. E.

AU - Clark, M. R.

N1 - Funding Information: Tissue specimens were obtained with the assistance of F.B.Askin, M.D., Department of Pathology, University of North Carolina at Chapel Hill. The technical assistance of Carole Bryant in preparing the luteal sections is gratefully appreciated. The research was supported by NIH grants HD-22299, HD-18968, and the Department of Obstetrics and Gynecology.

PY - 1990/10

Y1 - 1990/10

N2 - Prostaglandins have been implicated in both maintenance and luteolysis of the primate corpus luteum. Central to the production of prostaglandins is the enzyme prostaglandin endoperoxide synthase (PGHS). In the present study, we identified the cell types which contain PGHS in 44 human corpora lutea, using immunoperoxidase staining techniques. Intense granular staining was present in the cytoplasm of granulosa lutein cells of tissues obtained from the mid-luteal phase. Theca lutein cells demonstrated a diffuse cytoplasmic staining which was less intense than that observed in granulosa lutein cells. Staining appeared less intense in tisues from the early or late phase. Ovarian stromal cells demonstrated little or no PGHS immunoreactivity. PGHS staining in the corpus luteum of pregnancy was similar in intensity and cell distribution to that of mid-luted corpus luteum. In summary, human corpus luteum contains immunoreactive PGHS which localized mainly to welldifferentiated granulosa lutein cells.

AB - Prostaglandins have been implicated in both maintenance and luteolysis of the primate corpus luteum. Central to the production of prostaglandins is the enzyme prostaglandin endoperoxide synthase (PGHS). In the present study, we identified the cell types which contain PGHS in 44 human corpora lutea, using immunoperoxidase staining techniques. Intense granular staining was present in the cytoplasm of granulosa lutein cells of tissues obtained from the mid-luteal phase. Theca lutein cells demonstrated a diffuse cytoplasmic staining which was less intense than that observed in granulosa lutein cells. Staining appeared less intense in tisues from the early or late phase. Ovarian stromal cells demonstrated little or no PGHS immunoreactivity. PGHS staining in the corpus luteum of pregnancy was similar in intensity and cell distribution to that of mid-luted corpus luteum. In summary, human corpus luteum contains immunoreactive PGHS which localized mainly to welldifferentiated granulosa lutein cells.

KW - Corpus luteum

KW - Cyclooxygenase

KW - Immunohistochemistry

KW - Prostaglandin endoperoxide synthase

UR - https://www.scopus.com/pages/publications/0025116281

UR - https://www.scopus.com/pages/publications/0025116281#tab=citedBy

U2 - 10.1093/oxfordjournals.humrep.a137186

DO - 10.1093/oxfordjournals.humrep.a137186

M3 - Article

C2 - 2125055

AN - SCOPUS:0025116281

SN - 0268-1161

VL - 5

SP - 800

EP - 804

JO - Human Reproduction

JF - Human Reproduction

IS - 7

ER -

Localization of prostaglandin endoperoxide synthase in the human corpus luteum

Resumen

Nota bibliográfica

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ASJC Scopus subject areas

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