Manganese superoxide dismutase expression in human cancer cells: A possible role of mRNA processing

Daret K. St Clair; Larry W. Oberley

doi:10.3109/10715769109145858

Manganese superoxide dismutase expression in human cancer cells: A possible role of mRNA processing

Daret K. St Clair
, Larry W. Oberley

Producción científica: Article › revisión exhaustiva

23 Citas (Scopus)

Resumen

The level of manganese superoxide dismutase (MnSOD) as determined by an immunorcactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.

Idioma original	English
Páginas (desde-hasta)	771-778
Número de páginas	8
Publicación	Free Radical Research
Volumen	13
N.º	1
DOI	https://doi.org/10.3109/10715769109145858
Estado	Published - 1991

Nota bibliográfica

Funding Information:
This work was supported in part by a grant from the Forsyth Cancer Service, Inc., and NIH grants RR-0504. CA 41267. CA 12197-17 and CA 49797. The authors thank Pamela D. Cregger for preparation of this manuscript.

Financiación

This work was supported in part by a grant from the Forsyth Cancer Service, Inc., and NIH grants RR-0504. CA 41267. CA 12197-17 and CA 49797. The authors thank Pamela D. Cregger for preparation of this manuscript.

Financiadores	Número del financiador
Forsyth Cancer Service, Inc.
National Institutes of Health (NIH)	CA 41267, RR-0504
National Childhood Cancer Registry – National Cancer Institute	P30CA012197

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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ASJC Scopus subject areas

Biochemistry

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title = "Manganese superoxide dismutase expression in human cancer cells: A possible role of mRNA processing",

abstract = "The level of manganese superoxide dismutase (MnSOD) as determined by an immunorcactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.",

keywords = "Human cancer, MRNA processing, MnSOD, Northern blot, Western blot",

author = "\{St Clair\}, \{Daret K.\} and Oberley, \{Larry W.\}",

note = "Funding Information: This work was supported in part by a grant from the Forsyth Cancer Service, Inc., and NIH grants RR-0504. CA 41267. CA 12197-17 and CA 49797. The authors thank Pamela D. Cregger for preparation of this manuscript.",

year = "1991",

doi = "10.3109/10715769109145858",

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T1 - Manganese superoxide dismutase expression in human cancer cells

T2 - A possible role of mRNA processing

AU - St Clair, Daret K.

AU - Oberley, Larry W.

N1 - Funding Information: This work was supported in part by a grant from the Forsyth Cancer Service, Inc., and NIH grants RR-0504. CA 41267. CA 12197-17 and CA 49797. The authors thank Pamela D. Cregger for preparation of this manuscript.

PY - 1991

Y1 - 1991

N2 - The level of manganese superoxide dismutase (MnSOD) as determined by an immunorcactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.

AB - The level of manganese superoxide dismutase (MnSOD) as determined by an immunorcactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.

KW - Human cancer

KW - MRNA processing

KW - MnSOD

KW - Northern blot

KW - Western blot

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U2 - 10.3109/10715769109145858

DO - 10.3109/10715769109145858

M3 - Article

C2 - 2060849

AN - SCOPUS:84907037163

SN - 1071-5762

VL - 13

SP - 771

EP - 778

JO - Free Radical Research

JF - Free Radical Research

IS - 1

ER -

Manganese superoxide dismutase expression in human cancer cells: A possible role of mRNA processing

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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