Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson; Ramon C. Sun

doi:10.1007/978-1-0716-2030-4_15

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson
, Ramon C. Sun

Producción científica: Chapter › revisión exhaustiva

15 Citas (Scopus)

Resumen

Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

Idioma original	English
Título de la publicación alojada	Methods in Molecular Biology
Páginas	215-228
Número de páginas	14
DOI	https://doi.org/10.1007/978-1-0716-2030-4_15
Estado	Published - 2022

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	2437
ISSN (versión impresa)	1064-3745
ISSN (versión digital)	1940-6029

Nota bibliográfica

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Financiación

R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick’s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558.

Financiadores	Número del financiador
NIH-funded University of Kentucky Center for Cancer and Metabolism	P30CA177558
Departments of Urology, Pathology, The Markey Cancer Center and Department of Toxicology, University of Kentucky College of Medicine
National Institutes of Health (NIH)
National Institute on Aging	R01AG066653
National Institute on Aging
University of Kentucky Markey Cancer Center

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Molecular Biology
Genetics

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title = "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ",

abstract = "Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.",

keywords = "Alzheimer{\textquoteright}s disease, FFPE tissue, Glycogen, Glycogen chain length, Isoamylase, MALDI-MSI",

author = "Hawkinson, \{Tara R.\} and Sun, \{Ramon C.\}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

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language = "English",

series = "Methods in Molecular Biology",

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AU - Hawkinson, Tara R.

AU - Sun, Ramon C.

PY - 2022

Y1 - 2022

N2 - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

AB - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

KW - Alzheimer’s disease

KW - FFPE tissue

KW - Glycogen

KW - Glycogen chain length

KW - Isoamylase

KW - MALDI-MSI

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AN - SCOPUS:85121569422

T3 - Methods in Molecular Biology

SP - 215

EP - 228

BT - Methods in Molecular Biology

ER -

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Resumen

Serie de la publicación

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Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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