Measurement of Autotaxin/Lysophospholipase D Activity

Andrew J. Morris; Susan S. Smyth

doi:10.1016/S0076-6879(07)34005-6

Measurement of Autotaxin/Lysophospholipase D Activity

Andrew J. Morris, Susan S. Smyth

Internal Medicine

Producción científica: Chapter › revisión exhaustiva

14 Citas (Scopus)

Resumen

Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

Idioma original	English
Título de la publicación alojada	Lipidomics and Bioactive Lipids
Subtítulo de la publicación alojada	Lipids and Cell Signaling
Editores	Alex Brown
Páginas	89-104
Número de páginas	16
DOI	https://doi.org/10.1016/S0076-6879(07)34005-6
Estado	Published - 2007

Serie de la publicación

Nombre	Methods in Enzymology
Volumen	434
ISSN (versión impresa)	0076-6879

Nota bibliográfica

Funding Information:
Research in the authors' laboratories is supported by grants from the National Institutes of Health.

Financiación

Research in the authors' laboratories is supported by grants from the National Institutes of Health.

Financiadores	Número del financiador
National Institutes of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)	R01HL078663

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Acceder al documento

10.1016/S0076-6879(07)34005-6

Otros archivos y enlaces

Citar esto

@inbook{00012396e7ad48f081ee7b6090bb3d1a,

title = "Measurement of Autotaxin/Lysophospholipase D Activity",

abstract = "Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.",

author = "Morris, \{Andrew J.\} and Smyth, \{Susan S.\}",

note = "Funding Information: Research in the authors' laboratories is supported by grants from the National Institutes of Health.",

year = "2007",

doi = "10.1016/S0076-6879(07)34005-6",

language = "English",

isbn = "9780123739650",

series = "Methods in Enzymology",

pages = "89--104",

editor = "Alex Brown",

booktitle = "Lipidomics and Bioactive Lipids",

}

TY - CHAP

T1 - Measurement of Autotaxin/Lysophospholipase D Activity

AU - Morris, Andrew J.

AU - Smyth, Susan S.

N1 - Funding Information: Research in the authors' laboratories is supported by grants from the National Institutes of Health.

PY - 2007

Y1 - 2007

N2 - Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

AB - Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

UR - http://www.scopus.com/inward/record.url?scp=38449122263&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38449122263&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(07)34005-6

DO - 10.1016/S0076-6879(07)34005-6

M3 - Chapter

C2 - 17954243

AN - SCOPUS:38449122263

SN - 9780123739650

T3 - Methods in Enzymology

SP - 89

EP - 104

BT - Lipidomics and Bioactive Lipids

A2 - Brown, Alex

ER -

Measurement of Autotaxin/Lysophospholipase D Activity

Resumen

Serie de la publicación

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto