Measurement of binding kinetics using the gel electrophoresis mobility shift assay

The gel electrophoresis mobility shift assay is a technique for the qualitative and quantitative analysis of protein‐DNA complexes. The ability to resolve reactants, reaction intermediates and products makes this method particularly well‐suited for the measurement of the assembly and dissociation rates of protein‐nucleic acid complexes. Here we identify conditions that must be met and variations of the technique that are useful for the measurement of reaction rates.