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Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry

  • Maria P. Kraemer
  • , Suchismita Halder
  • , Susan S. Smyth
  • , Andrew J. Morris

Producción científica: Chapterrevisión exhaustiva

6 Citas (Scopus)

Resumen

Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

Idioma originalEnglish
Título de la publicación alojadaMethods in Molecular Biology
Páginas31-42
Número de páginas12
DOI
EstadoPublished - 2018

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1697
ISSN (versión impresa)1064-3745

Nota bibliográfica

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Financiación

FinanciadoresNúmero del financiador
U.S. Department of Veterans AffairsI01CX001550

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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