Measurements of cyclic AMP and cyclic GMP phosphodiesterase activity in isolated tubular segments

B. A. Jackson; R. M. Edwards; T. P. Dousa

doi:10.1038/ki.1980.166

Measurements of cyclic AMP and cyclic GMP phosphodiesterase activity in isolated tubular segments

B. A. Jackson
, R. M. Edwards
, T. P. Dousa

Producción científica: Article › revisión exhaustiva

16 Citas (Scopus)

Resumen

We have developed a method for the determination of both 3',5' - AMP phosphodiesterase and 3',5' - GMP phosphodiesterase activity in specific tubular segments microdissected from the rat kidney. In particular, we have applied this technique to measure the activity of these two enzymes in the medullary collecting tubule and the medullary thick ascending limb of the loop of Henle, two nephron segments recently shown to be major sites of VP-sensitive adenylate cyclase activity and responding to VP and cAMP by changes in water permeability or solute transport.

Idioma original	English
Páginas (desde-hasta)	512-518
Número de páginas	7
Publicación	Kidney International
Volumen	18
N.º	4
DOI	https://doi.org/10.1038/ki.1980.166
Estado	Published - 1980

Nota bibliográfica

Funding Information:
A preliminary report of this work was published in abstract form (Clin Res 26:780, 1978). This research was supported by USPHS NIH Grant AM-16105 from the National Institute of Arthritis, Me- tabolism and Digestive Diseases and by the Mayo Foundation. Dr. B. A. Jackson was the recipient of a Mayo Postdoctoral Research Fellowship and Wellcome Travel Award. Part of this research was con- ducted by R. M. Edwards in partial fulfillment of the requirements for the Ph.D. degree in Physiolo- gy, Mayo Graduate School of Medicine. Dr. T. P. Dousa, was an Established Investigator of the Ameri- can Heart Association (AHA-74182). Mrs. A. M. Walker provided secretarial assistance. Dr. D. Cha- bardes, Dr. M. Imbert-Teboul, and Prof. F. Morel, Laboratoire de Physiologie Cellulaire, College de France, Paris, France, gave advice in establishing tubule microdissection techniques.

Financiación

A preliminary report of this work was published in abstract form (Clin Res 26:780, 1978). This research was supported by USPHS NIH Grant AM-16105 from the National Institute of Arthritis, Me- tabolism and Digestive Diseases and by the Mayo Foundation. Dr. B. A. Jackson was the recipient of a Mayo Postdoctoral Research Fellowship and Wellcome Travel Award. Part of this research was con- ducted by R. M. Edwards in partial fulfillment of the requirements for the Ph.D. degree in Physiolo- gy, Mayo Graduate School of Medicine. Dr. T. P. Dousa, was an Established Investigator of the Ameri- can Heart Association (AHA-74182). Mrs. A. M. Walker provided secretarial assistance. Dr. D. Cha- bardes, Dr. M. Imbert-Teboul, and Prof. F. Morel, Laboratoire de Physiologie Cellulaire, College de France, Paris, France, gave advice in establishing tubule microdissection techniques.

Financiadores	Número del financiador
National Institute Arthritis Musculoskeletal & Skin
USPHS/NIH	AM-16105
Mayo Foundation for Medical Education and Research

ASJC Scopus subject areas

Nephrology

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abstract = "We have developed a method for the determination of both 3',5' - AMP phosphodiesterase and 3',5' - GMP phosphodiesterase activity in specific tubular segments microdissected from the rat kidney. In particular, we have applied this technique to measure the activity of these two enzymes in the medullary collecting tubule and the medullary thick ascending limb of the loop of Henle, two nephron segments recently shown to be major sites of VP-sensitive adenylate cyclase activity and responding to VP and cAMP by changes in water permeability or solute transport.",

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Measurements of cyclic AMP and cyclic GMP phosphodiesterase activity in isolated tubular segments

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

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