Measuring FRET efficiency with phase lifetime

Daniel Sumetsky; James Y. Jiang; Marina Ayad; Timothy Mahon; Audrey Menaesse; Marina M. Cararo-Lopes; Mihir V. Patel; Bonnie L. Firestein; Nada N. Boustany

Measuring FRET efficiency with phase lifetime

Daniel Sumetsky
, James Y. Jiang
, Marina Ayad
, Timothy Mahon
, Audrey Menaesse
, Marina M. Cararo-Lopes
, Mihir V. Patel
, Bonnie L. Firestein
, Nada N. Boustany

Pharmacology and Nutritional Sciences

Producción científica: Conference article › revisión exhaustiva

Resumen

A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

Idioma original	English
Número de artículo	MTu4A.4
Publicación	Optics InfoBase Conference Papers
Estado	Published - 2022
Evento	Microscopy Histopathology and Analytics, Microscopy 2022 - Fort Lauderdale, United States Duración: abr 24 2022 → abr 27 2022

Nota bibliográfica

Publisher Copyright:
© 2022 The Author(s).

Financiación

Acknowledgements: This study was supported by a NSF GOALI grant under award CMMI-1825433 to N.N.B and B.L.F. M.C.C. was supported by a Predoctoral Fellowship from CAPES, and M.P. was supported by NJCBIR Predoctoral Fellowship #CBIR15FEL009.

Financiadores	Número del financiador
National Science Foundation	CMMI-1825433
New Jersey Commission on Brain Injury Research	15FEL009
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

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@article{197fa86399a740258aeefadd21d2071c,

title = "Measuring FRET efficiency with phase lifetime",

abstract = "A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.",

author = "Daniel Sumetsky and Jiang, \{James Y.\} and Marina Ayad and Timothy Mahon and Audrey Menaesse and Cararo-Lopes, \{Marina M.\} and Patel, \{Mihir V.\} and Firestein, \{Bonnie L.\} and Boustany, \{Nada N.\}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s).; Microscopy Histopathology and Analytics, Microscopy 2022 ; Conference date: 24-04-2022 Through 27-04-2022",

year = "2022",

language = "English",

}

TY - JOUR

T1 - Measuring FRET efficiency with phase lifetime

AU - Sumetsky, Daniel

AU - Jiang, James Y.

AU - Ayad, Marina

AU - Mahon, Timothy

AU - Menaesse, Audrey

AU - Cararo-Lopes, Marina M.

AU - Patel, Mihir V.

AU - Firestein, Bonnie L.

AU - Boustany, Nada N.

PY - 2022

Y1 - 2022

N2 - A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

AB - A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

UR - https://www.scopus.com/pages/publications/85136440344

UR - https://www.scopus.com/pages/publications/85136440344#tab=citedBy

M3 - Conference article

AN - SCOPUS:85136440344

JO - Optics InfoBase Conference Papers

JF - Optics InfoBase Conference Papers

M1 - MTu4A.4

T2 - Microscopy Histopathology and Analytics, Microscopy 2022

Y2 - 24 April 2022 through 27 April 2022

ER -

Measuring FRET efficiency with phase lifetime

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Otros archivos y enlaces

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