Methodology to identify poly-ADP-ribose polymerase 1 (PARP1)–mRNA targets by PAR-CLiP

  • Manana Mekishvili
  • , Elena Matveeva
  • , Yvonne Fondufe-Mittendorf

Producción científica: Chapterrevisión exhaustiva

6 Citas (Scopus)

Resumen

There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA–protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing.

Idioma originalEnglish
Título de la publicación alojadaMethods in Molecular Biology
Páginas211-228
Número de páginas18
DOI
EstadoPublished - 2017

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1608
ISSN (versión impresa)1064-3745

Nota bibliográfica

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Financiación

FinanciadoresNúmero del financiador
National Institute of Environmental Health Sciences (NIEHS)R01ES024478

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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