Microarray-Based Identification of Transcription Factor Target Genes

Maartje Gorte; Anneke Horstman; Robert B. Page; Renze Heidstra; Arnold Stromberg; Kim Boutilier

doi:10.1007/978-1-61779-154-3_7

Microarray-Based Identification of Transcription Factor Target Genes

Maartje Gorte
, Anneke Horstman
, Robert B. Page
, Renze Heidstra
, Arnold Stromberg
, Kim Boutilier

Dr. Bing Zhang Department of Statistics

Producción científica: Chapter › revisión exhaustiva

4 Citas (Scopus)

Resumen

Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.

Idioma original	English
Título de la publicación alojada	Plant Transcription Factors
Subtítulo de la publicación alojada	Methods and Protocols
Páginas	119-141
Número de páginas	23
DOI	https://doi.org/10.1007/978-1-61779-154-3_7
Estado	Published - 2011

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	754
ISSN (versión impresa)	1064-3745
ISSN (versión digital)	1940-6029

Nota bibliográfica

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Financiación

M.G. was supported by a Netherlands Genomic Initiative Horizon grant. A.H. was supported by a Technology Top Institute Green Genetics grant. A.S. was supported by NIH P20 RR16481 and NSF EPS-0814194.

Financiadores	Número del financiador
National Science Foundation (NSF)	EPS-0814194
National Institutes of Health (NIH)	P20 RR16481

ASJC Scopus subject areas

Molecular Biology
Genetics

Acceder al documento

10.1007/978-1-61779-154-3_7

Otros archivos y enlaces

Citar esto

@inbook{51a3825bfd63431484b48b886de10a98,

title = "Microarray-Based Identification of Transcription Factor Target Genes",

abstract = "Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.",

keywords = "Microarray, cycloheximide, inducible gene expression systems, relative quantification, quantitative real-time RT-PCR, endogenous reference gene, amplification efficiency, target gene, transcription factor",

author = "Maartje Gorte and Anneke Horstman and Page, \{Robert B.\} and Renze Heidstra and Arnold Stromberg and Kim Boutilier",

note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",

year = "2011",

doi = "10.1007/978-1-61779-154-3\_7",

language = "English",

isbn = "9781617791536",

series = "Methods in Molecular Biology",

pages = "119--141",

booktitle = "Plant Transcription Factors",

}

TY - CHAP

T1 - Microarray-Based Identification of Transcription Factor Target Genes

AU - Gorte, Maartje

AU - Horstman, Anneke

AU - Page, Robert B.

AU - Heidstra, Renze

AU - Stromberg, Arnold

AU - Boutilier, Kim

PY - 2011

Y1 - 2011

N2 - Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.

AB - Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.

KW - Microarray

KW - cycloheximide

KW - inducible gene expression systems

KW - relative quantification, quantitative real-time RT-PCR, endogenous reference gene, amplification efficiency

KW - target gene

KW - transcription factor

UR - https://www.scopus.com/pages/publications/79960736391

UR - https://www.scopus.com/pages/publications/79960736391#tab=citedBy

U2 - 10.1007/978-1-61779-154-3_7

DO - 10.1007/978-1-61779-154-3_7

M3 - Chapter

C2 - 21720950

AN - SCOPUS:79960736391

SN - 9781617791536

T3 - Methods in Molecular Biology

SP - 119

EP - 141

BT - Plant Transcription Factors

ER -

Microarray-Based Identification of Transcription Factor Target Genes

Resumen

Serie de la publicación

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto