Molecular cloning, sequencing, and expression of equine interleukin-6

C. E. Swiderski; G. Sobol; D. P. Lunn; D. W. Horohov

doi:10.1016/S0165-2427(00)00241-5

Molecular cloning, sequencing, and expression of equine interleukin-6

C. E. Swiderski, G. Sobol, D. P. Lunn, D. W. Horohov

Veterinary Science

Producción científica: Article › revisión exhaustiva

11 Citas (Scopus)

Resumen

Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

Idioma original	English
Páginas (desde-hasta)	213-220
Número de páginas	8
Publicación	Veterinary Immunology and Immunopathology
Volumen	77
N.º	3-4
DOI	https://doi.org/10.1016/S0165-2427(00)00241-5
Estado	Published - dic 29 2000

Nota bibliográfica

Funding Information:
This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.

Financiación

This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.

Financiadores	Número del financiador
National Institutes of Health (NIH)
Grayson Jockey Club Research Foundation Inc
Louisiana State University

ASJC Scopus subject areas

Immunology
General Veterinary

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10.1016/S0165-2427(00)00241-5

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title = "Molecular cloning, sequencing, and expression of equine interleukin-6",

abstract = "Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84\% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.",

keywords = "Equine interleukin-6 (IL-6) cloning expression",

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AU - Swiderski, C. E.

AU - Sobol, G.

AU - Lunn, D. P.

AU - Horohov, D. W.

N1 - Funding Information: This work was supported by an NIH Idea award, the Grayson Jockey Club Research Foundation, and the Louisiana State University’s Equine Health Studies Program.

PY - 2000/12/29

Y1 - 2000/12/29

N2 - Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

AB - Equine interleukin-6 (IL-6) cDNA was amplified from mitogen-stimulated equine peripheral blood mononuclear cells (PBMC) using consensus sequence primers. The 727 bp amplified cDNA contains the entire coding region for equine IL-6 and includes 118 bases in the 3′ non-translated region. The coding sequence translates to a protein of 208 amino acids with a predicted 28 amino acid leader sequence. The mature protein of 180 amino acids has a predicted molecular mass of 20 471 Da without post-translational modifications. The amino acid sequence of equine IL-6 displays between 46 and 84% similarity to other mammalian IL-6 sequences. Expression of equine IL-6 in Chinese hamster ovary (CHO) cells yielded a supernatant that supported the proliferation of B9 cells in a dose-dependent manner. Treatment of B9 cells with an anti-IL-6 receptor antibody ablated the response to the recombinant equine IL-6.

KW - Equine interleukin-6 (IL-6) cloning expression

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JO - Veterinary Immunology and Immunopathology

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Molecular cloning, sequencing, and expression of equine interleukin-6

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