Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat “Triticurn aestivum L. 2n=42” and eastern gamagrass “Tripsacum dactyloides L. 2n=4x=72” are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides “male parent” specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.