Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Pavel Novak; Chao Li; Andrew I. Shevchuk; Ruben Stepanyan; Matthew Caldwell; Simon Hughes; Trevor G. Smart; Julia Gorelik; Victor P. Ostanin; Max J. Lab; Guy W.J. Moss; Gregory I. Frolenkov; David Klenerman; Yuri E. Korchev

doi:10.1038/nmeth.1306

Nanoscale live-cell imaging using hopping probe ion conductance microscopy

Pavel Novak
, Chao Li
, Andrew I. Shevchuk
, Ruben Stepanyan
, Matthew Caldwell
, Simon Hughes
, Trevor G. Smart
, Julia Gorelik
, Victor P. Ostanin
, Max J. Lab
, Guy W.J. Moss
, Gregory I. Frolenkov
, David Klenerman
, Yuri E. Korchev

Physiology

Producción científica: Article › revisión exhaustiva

475 Citas (Scopus)

Resumen

We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

Idioma original	English
Páginas (desde-hasta)	279-281
Número de páginas	3
Publicación	Nature Methods
Volumen	6
N.º	4
DOI	https://doi.org/10.1038/nmeth.1306
Estado	Published - 2009

Nota bibliográfica

Funding Information:
This study was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council to Y.E.K. and (BB/D01817X/1) to G.W.J.M. and T.G.S., and by the US National Organization for Hearing Research Foundation, the Kentucky Science and Engineering Foundation (KSEF-148-502-07-215) and National Institute on Deafness and Other Communication Disorders, National Institutes of Health (DC008861 to G.I.F.).

Financiación

This study was supported by the UK Biotechnology and Biological Sciences Research Council and Medical Research Council to Y.E.K. and (BB/D01817X/1) to G.W.J.M. and T.G.S., and by the US National Organization for Hearing Research Foundation, the Kentucky Science and Engineering Foundation (KSEF-148-502-07-215) and National Institute on Deafness and Other Communication Disorders, National Institutes of Health (DC008861 to G.I.F.).

Financiadores	Número del financiador
National Organization for Hearing Research Foundation
National Institutes of Health (NIH)
Biotechnology and Biological Sciences Research Council	BB/D01817X/1, BB/D020816/1, BB/D018595/1, BB/D020875/1
Medical Research Council-São Paulo Research Foundation	G0601529, BB/D01817X/1
Kentucky Science and Engineering Foundation	KSEF-148-502-07-215
National Institute on Deafness and Other Communication Disorders	R01DC008861

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "Nanoscale live-cell imaging using hopping probe ion conductance microscopy",

abstract = "We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.",

author = "Pavel Novak and Chao Li and Shevchuk, \{Andrew I.\} and Ruben Stepanyan and Matthew Caldwell and Simon Hughes and Smart, \{Trevor G.\} and Julia Gorelik and Ostanin, \{Victor P.\} and Lab, \{Max J.\} and Moss, \{Guy W.J.\} and Frolenkov, \{Gregory I.\} and David Klenerman and Korchev, \{Yuri E.\}",

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AU - Li, Chao

AU - Shevchuk, Andrew I.

AU - Stepanyan, Ruben

AU - Caldwell, Matthew

AU - Hughes, Simon

AU - Smart, Trevor G.

AU - Gorelik, Julia

AU - Ostanin, Victor P.

AU - Lab, Max J.

AU - Moss, Guy W.J.

AU - Frolenkov, Gregory I.

AU - Klenerman, David

AU - Korchev, Yuri E.

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PY - 2009

Y1 - 2009

N2 - We describe hopping mode scanning ion conductance microscopy that allows noncontact imaging of the complex three-dimensional surfaces of live cells with resolution better than 20 nm. We tested the effectiveness of this technique by imaging networks of cultured rat hippocampal neurons and mechanosensory stereocilia of mouse cochlear hair cells. The technique allowed examination of nanoscale phenomena on the surface of live cells under physiological conditions.

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DO - 10.1038/nmeth.1306

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Nanoscale live-cell imaging using hopping probe ion conductance microscopy

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Nota bibliográfica

Financiación

ASJC Scopus subject areas

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