TY - JOUR
T1 - Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon
AU - Gounaris, Elias
AU - Ishihara, Yasushige
AU - Shrivastrav, Manisha
AU - Bentrem, David
AU - Barrett, Terrence A.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016
Y1 - 2016
N2 - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.
AB - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.
KW - Cathepsin activity
KW - Colon cancer
KW - Endoscopy
KW - Mouse colon dysplasia
KW - Mucosal inflammation
KW - Near-infrared probes
UR - http://www.scopus.com/inward/record.url?scp=84990060834&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-3603-8_13
DO - 10.1007/978-1-4939-3603-8_13
M3 - Article
C2 - 27246029
AN - SCOPUS:84990060834
SN - 1064-3745
VL - 1422
SP - 137
EP - 147
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -
