Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)

Fredrik Barrenas; Richard R. Green; Matthew J. Thomas; G. Lynn Law; Sean C. Proll; Flora Engelmann; Ilhem Messaoudi; Andrea Marzi; Heinz Feldmann; Michael G. Katzea

doi:10.1128/CVI.00733-14

Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)

Fredrik Barrenas
, Richard R. Green
, Matthew J. Thomas
, G. Lynn Law
, Sean C. Proll
, Flora Engelmann
, Ilhem Messaoudi
, Andrea Marzi
, Heinz Feldmann
, Michael G. Katzea

Producción científica: Article › revisión exhaustiva

16 Citas (Scopus)

Resumen

Vesicularstomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.

Idioma original	English
Páginas (desde-hasta)	354-356
Número de páginas	3
Publicación	Clinical and Vaccine Immunology
Volumen	22
N.º	3
DOI	https://doi.org/10.1128/CVI.00733-14
Estado	Published - mar 1 2015

Nota bibliográfica

Publisher Copyright:
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Financiación

Financiadores	Número del financiador
National Institute of Allergy and Infectious Diseases	ZIAAI001163

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

Acceder al documento

10.1128/CVI.00733-14

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Profundice en los temas de investigación de 'Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)'. En conjunto forman una huella única.

Citar esto

Barrenas, F., Green, R. R., Thomas, M. J., Law, G. L., Proll, S. C., Engelmann, F., Messaoudi, I., Marzi, A., Feldmann, H., & Katzea, M. G. (2015). Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). Clinical and Vaccine Immunology, 22(3), 354-356. https://doi.org/10.1128/CVI.00733-14

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abstract = "Vesicularstomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.",

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Barrenas, F, Green, RR, Thomas, MJ, Law, GL, Proll, SC, Engelmann, F, Messaoudi, I, Marzi, A, Feldmann, H & Katzea, MG 2015, 'Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp)', Clinical and Vaccine Immunology, vol. 22, n.º 3, pp. 354-356. https://doi.org/10.1128/CVI.00733-14

Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). / Barrenas, Fredrik; Green, Richard R.; Thomas, Matthew J. et al.
En: Clinical and Vaccine Immunology, Vol. 22, N.º 3, 01.03.2015, p. 354-356.

Producción científica: Article › revisión exhaustiva

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AU - Law, G. Lynn

AU - Proll, Sean C.

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AU - Feldmann, Heinz

AU - Katzea, Michael G.

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Barrenas F, Green RR, Thomas MJ, Law GL, Proll SC, Engelmann F et al. Next-Generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp). Clinical and Vaccine Immunology. 2015 mar 1;22(3):354-356. doi: 10.1128/CVI.00733-14