Nitrate-nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola)

Lisa J. Vaillancourt; Robert M. Hanau

doi:10.1016/S0147-5975(06)80004-6

Nitrate-nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola)

Lisa J. Vaillancourt
, Robert M. Hanau

Producción científica: Article › revisión exhaustiva

17 Citas (Scopus)

Resumen

Nitrate-nonutilizing (nit) mutants of Glomerella graminicola were recovered by selecting chlorate-resistant sectors. Heterokaryons were formed by complementation between two different classes of nit mutants. Complementation groups were distinguished in nitrogen feeding tests and segregated as two, unlinked genes among random progeny of sexual crosses. The two genes are comparable to those encoding the nitrate reductase enzyme and one of a series of molybdenum cofactors in Aspergillus nidulans and Neurospora crassa. Heterokaryon tests were reliable indicators of allelic and dominance relationships between mutations with similar phenotypes. Vegetative compatibility (VC) between two strains of G. graminicola appeared to be regulated by approximately five unlinked VC loci, analogous to those described for other fungi.

Idioma original	English
Páginas (desde-hasta)	311-319
Número de páginas	9
Publicación	Experimental Mycology
Volumen	18
N.º	4
DOI	https://doi.org/10.1016/S0147-5975(06)80004-6
Estado	Published - 1994

Nota bibliográfica

Funding Information:
We are grateful to Dr. Ralph Nicholson and Dr. H. P. da Silva for generously providing some of the fungal isolates used in this study. We especially thank Mr. Howard Laffoon for excellent technical assistance. These studies were supported by Grant 90-37262-5445 from the CRGO of the USDA and by tri-agency Grant 92-37310-7821 from the DOE/NSF/ USDA for joint programs on collaborative research in plant biology. This is Journal Paper 13,974 from the Purdue University Agricultural Experiment Station.

Financiación

We are grateful to Dr. Ralph Nicholson and Dr. H. P. da Silva for generously providing some of the fungal isolates used in this study. We especially thank Mr. Howard Laffoon for excellent technical assistance. These studies were supported by Grant 90-37262-5445 from the CRGO of the USDA and by tri-agency Grant 92-37310-7821 from the DOE/NSF/ USDA for joint programs on collaborative research in plant biology. This is Journal Paper 13,974 from the Purdue University Agricultural Experiment Station.

Financiadores	Número del financiador
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China
U.S. Department of Energy Oak Ridge National Laboratory U.S. Department of Energy National Science Foundation National Energy Research Scientific Computing Center
U.S. Department of Agriculture	92-37310-7821
U.S. Department of Agriculture

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1016/S0147-5975(06)80004-6

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title = "Nitrate-nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola)",

abstract = "Nitrate-nonutilizing (nit) mutants of Glomerella graminicola were recovered by selecting chlorate-resistant sectors. Heterokaryons were formed by complementation between two different classes of nit mutants. Complementation groups were distinguished in nitrogen feeding tests and segregated as two, unlinked genes among random progeny of sexual crosses. The two genes are comparable to those encoding the nitrate reductase enzyme and one of a series of molybdenum cofactors in Aspergillus nidulans and Neurospora crassa. Heterokaryon tests were reliable indicators of allelic and dominance relationships between mutations with similar phenotypes. Vegetative compatibility (VC) between two strains of G. graminicola appeared to be regulated by approximately five unlinked VC loci, analogous to those described for other fungi.",

keywords = "Glomerella graminicola, Mel, VC, anthracnose, chlorate resistance, heterokaryosis, melanin-deficient, nit, nitrate reductase, nitrate-nonutilizing, vegetative compatibility, vegetative compatibility",

author = "Vaillancourt, \{Lisa J.\} and Hanau, \{Robert M.\}",

note = "Funding Information: We are grateful to Dr. Ralph Nicholson and Dr. H. P. da Silva for generously providing some of the fungal isolates used in this study. We especially thank Mr. Howard Laffoon for excellent technical assistance. These studies were supported by Grant 90-37262-5445 from the CRGO of the USDA and by tri-agency Grant 92-37310-7821 from the DOE/NSF/ USDA for joint programs on collaborative research in plant biology. This is Journal Paper 13,974 from the Purdue University Agricultural Experiment Station.",

year = "1994",

doi = "10.1016/S0147-5975(06)80004-6",

language = "English",

volume = "18",

pages = "311--319",

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AU - Vaillancourt, Lisa J.

AU - Hanau, Robert M.

N1 - Funding Information: We are grateful to Dr. Ralph Nicholson and Dr. H. P. da Silva for generously providing some of the fungal isolates used in this study. We especially thank Mr. Howard Laffoon for excellent technical assistance. These studies were supported by Grant 90-37262-5445 from the CRGO of the USDA and by tri-agency Grant 92-37310-7821 from the DOE/NSF/ USDA for joint programs on collaborative research in plant biology. This is Journal Paper 13,974 from the Purdue University Agricultural Experiment Station.

PY - 1994

Y1 - 1994

N2 - Nitrate-nonutilizing (nit) mutants of Glomerella graminicola were recovered by selecting chlorate-resistant sectors. Heterokaryons were formed by complementation between two different classes of nit mutants. Complementation groups were distinguished in nitrogen feeding tests and segregated as two, unlinked genes among random progeny of sexual crosses. The two genes are comparable to those encoding the nitrate reductase enzyme and one of a series of molybdenum cofactors in Aspergillus nidulans and Neurospora crassa. Heterokaryon tests were reliable indicators of allelic and dominance relationships between mutations with similar phenotypes. Vegetative compatibility (VC) between two strains of G. graminicola appeared to be regulated by approximately five unlinked VC loci, analogous to those described for other fungi.

AB - Nitrate-nonutilizing (nit) mutants of Glomerella graminicola were recovered by selecting chlorate-resistant sectors. Heterokaryons were formed by complementation between two different classes of nit mutants. Complementation groups were distinguished in nitrogen feeding tests and segregated as two, unlinked genes among random progeny of sexual crosses. The two genes are comparable to those encoding the nitrate reductase enzyme and one of a series of molybdenum cofactors in Aspergillus nidulans and Neurospora crassa. Heterokaryon tests were reliable indicators of allelic and dominance relationships between mutations with similar phenotypes. Vegetative compatibility (VC) between two strains of G. graminicola appeared to be regulated by approximately five unlinked VC loci, analogous to those described for other fungi.

KW - Glomerella graminicola

KW - Mel

KW - VC

KW - anthracnose

KW - chlorate resistance

KW - heterokaryosis

KW - melanin-deficient

KW - nit

KW - nitrate reductase

KW - nitrate-nonutilizing

KW - vegetative compatibility

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U2 - 10.1016/S0147-5975(06)80004-6

DO - 10.1016/S0147-5975(06)80004-6

M3 - Article

AN - SCOPUS:0028558743

SN - 0147-5975

VL - 18

SP - 311

EP - 319

JO - Experimental Mycology

JF - Experimental Mycology

IS - 4

ER -

Nitrate-nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola (Colletotrichum graminicola)

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Nota bibliográfica

Financiación

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