Nonparametric statistical inference for multivariate niche overlap

In ecological studies niche overlap is often used to quantify species interaction and dynamics. This paper develops a robust, nonparametric statistical framework for quantifying and analyzing multivariate niche overlap. Parametric methods are often constrained by restrictive assumptions and tend to underperform in complex multivariate settings. We introduce a nonparametric overlap index and propose estimators for it. Further, we investigate asymptotic properties of the estimators. We also propose bootstrap-based inference procedures that enable statistical testing and simultaneous confidence intervals in small sample settings. Extensive numerical examples demonstrate that our proposed methods maintain correct size and exhibit robust power across various scenarios. We illustrate the practical utility of our methodology using stable isotope measurements from multiple fish species and provide distinct ecological insights regarding species niche differentiation.