TY - GEN
T1 - Oriented assembly of proteins on surfaces
AU - Bachas, Leonidas G.
AU - Bhattacharyya, D.
AU - Anderson, Kimberly W.
PY - 1999
Y1 - 1999
N2 - Several approaches that facilitate the oriented assembly of proteins on a variety of surfaces, including membranes, will be described. Biotinylation reactions performed under controlled conditions were used to immobilize enzymes in a layer-by-layer fashion onto surfaces modified with avidin or streptavidin. For other enzymes, a specific attachment site was introduced by gene fusion or site-directed mutagenesis. These strategies were applied to achieve site-specific immobilization of several enzymes including alkaline phosphatase, organophosphorus hydrolase, horseradish peroxidase, and subtilisin. The site-specific immobilization led to orientation of the enzyme molecules on the surface of materials and to a higher activity compared to conventional immobilization methods.
AB - Several approaches that facilitate the oriented assembly of proteins on a variety of surfaces, including membranes, will be described. Biotinylation reactions performed under controlled conditions were used to immobilize enzymes in a layer-by-layer fashion onto surfaces modified with avidin or streptavidin. For other enzymes, a specific attachment site was introduced by gene fusion or site-directed mutagenesis. These strategies were applied to achieve site-specific immobilization of several enzymes including alkaline phosphatase, organophosphorus hydrolase, horseradish peroxidase, and subtilisin. The site-specific immobilization led to orientation of the enzyme molecules on the surface of materials and to a higher activity compared to conventional immobilization methods.
UR - https://www.scopus.com/pages/publications/0033344319
UR - https://www.scopus.com/pages/publications/0033344319#tab=citedBy
M3 - Conference contribution
AN - SCOPUS:0033344319
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 739
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -