TY - JOUR
T1 - Ovulations in rat ovaries perfused in vitro with follicle-stimulating hormone
AU - Shaykh, M.
AU - LeMaire, W. J.
AU - Papkoff, H.
AU - Curry, T. E.
AU - Sogn, J. H.
AU - Koos, R. D.
PY - 1985
Y1 - 1985
N2 - Using the model of the isolated perfused rat ovary, we have found that highly purified ovine follicle-stimulating hormone (FSH) preparations cause ovulation and that this effect is not due to luteinizing hormone induced contamination. Ovine FSH-13 at a concentration of 1.5 mU/ml (induced ovulations in all perfused ovaries (8.8±2.3 ovulations/ovary, as did a more purified preparation, ovine FSH-211B, at concentrations of 0.5mU/ml (15.0±6.4 ovulations/ovary) and 5mU/ml (11.3±2.6 ovulations/ovary). This ovulation-inducing effect of FSH is accompanied by a marked stimulation of estradiol levels in the perfusion medium without stimulation of progesterone levels. Furthermore, a purified rat FSH preparation (15 mU/ml also induced ovulation in all ovaries (13.8±2.2 ovulations/ovary) as well as a stimulation of both estradiol and progesterone in the medium. These data clearly confirm the direct ovulatory effect of FSH on the ovary.
AB - Using the model of the isolated perfused rat ovary, we have found that highly purified ovine follicle-stimulating hormone (FSH) preparations cause ovulation and that this effect is not due to luteinizing hormone induced contamination. Ovine FSH-13 at a concentration of 1.5 mU/ml (induced ovulations in all perfused ovaries (8.8±2.3 ovulations/ovary, as did a more purified preparation, ovine FSH-211B, at concentrations of 0.5mU/ml (15.0±6.4 ovulations/ovary) and 5mU/ml (11.3±2.6 ovulations/ovary). This ovulation-inducing effect of FSH is accompanied by a marked stimulation of estradiol levels in the perfusion medium without stimulation of progesterone levels. Furthermore, a purified rat FSH preparation (15 mU/ml also induced ovulation in all ovaries (13.8±2.2 ovulations/ovary) as well as a stimulation of both estradiol and progesterone in the medium. These data clearly confirm the direct ovulatory effect of FSH on the ovary.
UR - https://www.scopus.com/pages/publications/0022252966
UR - https://www.scopus.com/pages/publications/0022252966#tab=citedBy
U2 - 10.1095/biolreprod33.3.629
DO - 10.1095/biolreprod33.3.629
M3 - Article
C2 - 3931710
AN - SCOPUS:0022252966
SN - 0006-3363
VL - 33
SP - 629
EP - 636
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 3
ER -