Phage display library screening for identifi cation of interacting protein partners

Phage display is a versatile high-throughput screening method employed to understand and improve the chemical biology, be it production of human monoclonal antibodies or identifi cation of interacting protein partners. A majority of cell proteins operate in a concerted fashion either by stable or transient interactions. Such interactions can be mediated by recognition of small amino acid sequence motifs on the protein surface. Phage display can play a crucial role in identifi cation of such motifs. This report describes the use of phage display for the identifi cation of high affi nity sequence motifs that could be responsible for interactions with a target (bait) protein.