Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Gah Hyun Lim; M. B. Shine; Laura De Lorenzo; Keshun Yu; Weier Cui; Duroy Navarre; Arthur G. Hunt; Jung Youn Lee; Aardra Kachroo; Pradeep Kachroo

doi:10.1016/j.chom.2016.03.006

Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Gah Hyun Lim, M. B. Shine, Laura De Lorenzo, Keshun Yu, Weier Cui, Duroy Navarre, Arthur G. Hunt, Jung Youn Lee, Aardra Kachroo, Pradeep Kachroo

Producción científica: Article › revisión exhaustiva

143 Citas (Scopus)

Resumen

Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

Idioma original	English
Páginas (desde-hasta)	541-549
Número de páginas	9
Publicación	Cell Host and Microbe
Volumen	19
N.º	4
DOI	https://doi.org/10.1016/j.chom.2016.03.006
Estado	Published - abr 13 2016

Nota bibliográfica

Publisher Copyright:
© 2016 Elsevier Inc.

Financiación

This work was supported by grants from the National Science Foundation ( IOS#0749731 , #1457121 , #0954931 ) and a NSF grant under Cooperative Agreement No. 1355438 . We thank Andrea Pitzschke for AZI1-MYC transgenic plants, Arabidopsis database for PDLP knockout seeds, Xiangnan Guan for help with isolation of pdlp mutants, John Johnson for help with gas chromatography, Timothy Hoey for genotyping, Wendy Havens and Ludmila Lapchyk for technical help, and Amy Crume for managing the plant growth facility.

Financiadores	Número del financiador
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	IOS#0749731, 1243849, 1457121, 1355438, 0954931
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China

ASJC Scopus subject areas

Parasitology
Microbiology
Virology

Acceder al documento

10.1016/j.chom.2016.03.006

Otros archivos y enlaces

Citar esto

@article{505455bf1b88420ca307bfbae71885dc,

title = "Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants",

abstract = "Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.",

author = "Lim, \{Gah Hyun\} and Shine, \{M. B.\} and \{De Lorenzo\}, Laura and Keshun Yu and Weier Cui and Duroy Navarre and Hunt, \{Arthur G.\} and Lee, \{Jung Youn\} and Aardra Kachroo and Pradeep Kachroo",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2016",

month = apr,

day = "13",

doi = "10.1016/j.chom.2016.03.006",

language = "English",

volume = "19",

pages = "541--549",

number = "4",

}

TY - JOUR

T1 - Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

AU - Lim, Gah Hyun

AU - Shine, M. B.

AU - De Lorenzo, Laura

AU - Yu, Keshun

AU - Cui, Weier

AU - Navarre, Duroy

AU - Hunt, Arthur G.

AU - Lee, Jung Youn

AU - Kachroo, Aardra

AU - Kachroo, Pradeep

PY - 2016/4/13

Y1 - 2016/4/13

N2 - Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

AB - Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA- and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR.

UR - https://www.scopus.com/pages/publications/84963624207

UR - https://www.scopus.com/inward/citedby.url?scp=84963624207&partnerID=8YFLogxK

U2 - 10.1016/j.chom.2016.03.006

DO - 10.1016/j.chom.2016.03.006

M3 - Article

C2 - 27078071

AN - SCOPUS:84963624207

SN - 1931-3128

VL - 19

SP - 541

EP - 549

JO - Cell Host and Microbe

JF - Cell Host and Microbe

IS - 4

ER -

Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto