Primary tumor microbiomes predict distant metastasis of colorectal cancer

Bishal Parajuli; Vishal Midya; Ryan Kiddle; Nicola De Jager; Shoshannah Eggers; Daniel Spakowicz; Rebecca Hoyd; Bodour Salhia; Carlos H.F. Chan; Michelle L. Churchman; Robert J. Rounbehler; Song Yao; Melanie R. Rutkowski; Ahmad A. Tarhini; Dinesh Pal Mudaranthakam; Ashiq Masood; Therese J. Bocklage; Robert W. Lentz; Hassan Hatoum; Mmadili N. Ilozumba; Sheetal Hardikar; Cornelia M. Ulrich; June L. Round; Gregory Riedlinger; Craig D. Shriver; Dustin E. Bosch

doi:10.1038/s41698-025-01188-x

Primary tumor microbiomes predict distant metastasis of colorectal cancer

Bishal Parajuli
, Vishal Midya
, Ryan Kiddle
, Nicola De Jager
, Shoshannah Eggers
, Daniel Spakowicz
, Rebecca Hoyd
, Bodour Salhia
, Carlos H.F. Chan
, Michelle L. Churchman
, Robert J. Rounbehler
, Song Yao
, Melanie R. Rutkowski
, Ahmad A. Tarhini
, Dinesh Pal Mudaranthakam
, Ashiq Masood
, Therese J. Bocklage
, Robert W. Lentz
, Hassan Hatoum
, Mmadili N. Ilozumba

Sheetal Hardikar, Cornelia M. Ulrich, June L. Round, Gregory Riedlinger, Craig D. Shriver, Dustin E. Bosch

Producción científica: Article › revisión exhaustiva

3 Citas (Scopus)

Resumen

Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks + E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4–2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.

Idioma original	English
Número de artículo	405
Publicación	npj Precision Oncology
Volumen	9
N.º	1
DOI	https://doi.org/10.1038/s41698-025-01188-x
Estado	Published - dic 2025

Nota bibliográfica

Publisher Copyright:
© The Author(s) 2025.

Financiación

We gratefully acknowledge the University of Iowa Holden Comprehensive Cancer Center’s Microbiome Core for their essential support in providing microbiome sequencing services. The Histology Research Laboratory at Iowa provided histology services. This work was supported by the Holden Comprehensive Cancer Center ACS-IRG from the American Cancer Society (DEB). DEB was supported by the NIH, K08AI159619. RK was supported by the American Cancer Society (IRG-21-141-46-IRG) and the National Cancer Institute (R25CA273964). DS is supported by the National Institute on Aging (K01AG070310), the American Lung Association (1046611), and the American Cancer Society (RSG-23-1023205).

Financiadores	Número del financiador
American Cancer Society-Michigan Cancer Research Fund
Division of Environmental Biology
Iowa Environmental Mesonet at Iowa State University
National Childhood Cancer Registry – National Cancer Institute	R25CA273964
National Institutes of Health (NIH)	K08AI159619, IRG-21-141-46-IRG
National Institute on Aging	K01AG070310
American Lung Association	1046611, RSG-23-1023205

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Oncology
Cancer Research

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Parajuli, B., Midya, V., Kiddle, R., De Jager, N., Eggers, S., Spakowicz, D., Hoyd, R., Salhia, B., Chan, C. H. F., Churchman, M. L., Rounbehler, R. J., Yao, S., Rutkowski, M. R., Tarhini, A. A., Mudaranthakam, D. P., Masood, A., Bocklage, T. J., Lentz, R. W., Hatoum, H., ... Bosch, D. E. (2025). Primary tumor microbiomes predict distant metastasis of colorectal cancer. npj Precision Oncology, 9(1), Artículo 405. https://doi.org/10.1038/s41698-025-01188-x

@article{12fc4092550f446090f746dd9b28e60e,

title = "Primary tumor microbiomes predict distant metastasis of colorectal cancer",

abstract = "Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks + E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4–2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.",

author = "Bishal Parajuli and Vishal Midya and Ryan Kiddle and \{De Jager\}, Nicola and Shoshannah Eggers and Daniel Spakowicz and Rebecca Hoyd and Bodour Salhia and Chan, \{Carlos H.F.\} and Churchman, \{Michelle L.\} and Rounbehler, \{Robert J.\} and Song Yao and Rutkowski, \{Melanie R.\} and Tarhini, \{Ahmad A.\} and Mudaranthakam, \{Dinesh Pal\} and Ashiq Masood and Bocklage, \{Therese J.\} and Lentz, \{Robert W.\} and Hassan Hatoum and Ilozumba, \{Mmadili N.\} and Sheetal Hardikar and Ulrich, \{Cornelia M.\} and Round, \{June L.\} and Gregory Riedlinger and Shriver, \{Craig D.\} and Bosch, \{Dustin E.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",

year = "2025",

month = dec,

doi = "10.1038/s41698-025-01188-x",

language = "English",

volume = "9",

number = "1",

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Parajuli, B, Midya, V, Kiddle, R, De Jager, N, Eggers, S, Spakowicz, D, Hoyd, R, Salhia, B, Chan, CHF, Churchman, ML, Rounbehler, RJ, Yao, S, Rutkowski, MR, Tarhini, AA, Mudaranthakam, DP, Masood, A, Bocklage, TJ, Lentz, RW, Hatoum, H, Ilozumba, MN, Hardikar, S, Ulrich, CM, Round, JL, Riedlinger, G, Shriver, CD & Bosch, DE 2025, 'Primary tumor microbiomes predict distant metastasis of colorectal cancer', npj Precision Oncology, vol. 9, n.º 1, 405. https://doi.org/10.1038/s41698-025-01188-x

TY - JOUR

T1 - Primary tumor microbiomes predict distant metastasis of colorectal cancer

AU - Parajuli, Bishal

AU - Midya, Vishal

AU - Kiddle, Ryan

AU - De Jager, Nicola

AU - Eggers, Shoshannah

AU - Spakowicz, Daniel

AU - Hoyd, Rebecca

AU - Salhia, Bodour

AU - Chan, Carlos H.F.

AU - Churchman, Michelle L.

AU - Rounbehler, Robert J.

AU - Yao, Song

AU - Rutkowski, Melanie R.

AU - Tarhini, Ahmad A.

AU - Mudaranthakam, Dinesh Pal

AU - Masood, Ashiq

AU - Bocklage, Therese J.

AU - Lentz, Robert W.

AU - Hatoum, Hassan

AU - Ilozumba, Mmadili N.

AU - Hardikar, Sheetal

AU - Ulrich, Cornelia M.

AU - Round, June L.

AU - Riedlinger, Gregory

AU - Shriver, Craig D.

AU - Bosch, Dustin E.

PY - 2025/12

Y1 - 2025/12

N2 - Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks + E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4–2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.

AB - Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks + E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4–2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.

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UR - https://www.scopus.com/pages/publications/105026265008#tab=citedBy

U2 - 10.1038/s41698-025-01188-x

DO - 10.1038/s41698-025-01188-x

M3 - Article

AN - SCOPUS:105026265008

VL - 9

JO - npj Precision Oncology

JF - npj Precision Oncology

IS - 1

M1 - 405

ER -

Primary tumor microbiomes predict distant metastasis of colorectal cancer

Resumen

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Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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