Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Debora Lopes Salles Scheffel; Jaime Aparecido Cury; Livia Maria Andaló Tenuta; Régis Henke Scheffel; Cristina Perez; Diana Gabriela Soares; Fernanda Gonçalves Basso; Carlos Alberto de Souza Costa; David Henry Pashley; Josimeri Hebling

doi:10.1111/ipd.12632

Proteolytic activity, degradation, and dissolution of primary and permanent teeth

Debora Lopes Salles Scheffel
, Jaime Aparecido Cury
, Livia Maria Andaló Tenuta
, Régis Henke Scheffel
, Cristina Perez
, Diana Gabriela Soares
, Fernanda Gonçalves Basso
, Carlos Alberto de Souza Costa
, David Henry Pashley
, Josimeri Hebling

Oral Health Science

Producción científica: Article › revisión exhaustiva

12 Citas (Scopus)

Resumen

Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

Idioma original	English
Páginas (desde-hasta)	650-659
Número de páginas	10
Publicación	International Journal of Paediatric Dentistry
Volumen	30
N.º	5
DOI	https://doi.org/10.1111/ipd.12632
Estado	Published - sept 1 2020

Nota bibliográfica

Publisher Copyright:
© 2020 BSPD, IAPD and John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Financiación

The authors thank Ms Kelli Ann Agee and Mr Waldomiro Vieira Filho for their valuable technical support. This work was supported in part by the S?o Paulo Research Foundation (FAPESP), grants 2008/02278-8; 2009/07924-8; 2014/17232-4 and 2014/18160-7, by Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior?Brasil (CAPES)? Finance Code 693711-0, and by R01 DE05306 from the National Institute of Dental and Craniofacial Research (NIDCR). The authors thank Ms Kelli Ann Agee and Mr Waldomiro Vieira Filho for their valuable technical support. This work was supported in part by the São Paulo Research Foundation (FAPESP), grants 2008/02278‐8; 2009/07924‐8; 2014/17232‐4 and 2014/18160‐7, by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)— Finance Code 693711‐0, and by R01 DE05306 from the National Institute of Dental and Craniofacial Research (NIDCR).

Financiadores	Número del financiador
S?o Paulo Research Foundation
National Institute of Dental and Craniofacial Research
Fundação de Amparo à Pesquisa do Estado de São Paulo	2008/02278‐8, 2009/07924‐8, 2014/17232‐4, 2014/18160‐7
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	R01 DE05306

ASJC Scopus subject areas

General Dentistry

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title = "Proteolytic activity, degradation, and dissolution of primary and permanent teeth",

abstract = "Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.",

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AU - Scheffel, Debora Lopes Salles

AU - Cury, Jaime Aparecido

AU - Tenuta, Livia Maria Andaló

AU - Scheffel, Régis Henke

AU - Perez, Cristina

AU - Soares, Diana Gabriela

AU - Basso, Fernanda Gonçalves

AU - Costa, Carlos Alberto de Souza

AU - Pashley, David Henry

AU - Hebling, Josimeri

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

AB - Background: Primary and permanent teeth composition may influence dissolution and degradation rates. Aim: To compare the dissolution and degradation of primary and permanent teeth. Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers. Calcium and inorganic phosphate release was quantified in the buffers by atomic absorption and light spectrophotometry. A colorimetric assay was used to assess the MMP activity of primary dentin (PrD) and permanent dentin (PeD). Collagen degradation was assessed by dry mass loss, change in elastic modulus (E), and ICTP and CTX release. Data were submitted to ANOVA and Tukey's tests (α = 0.05). Results: Similar dissolution was found between PrD and PeD after 256 hours. At pH 4.5, enamel released more minerals than dentin whereas at pH 5.5 the inverse result was observed. MMP activity was similar for both substrates. PrD showed higher dry mass loss after 1 week. In general, greater reduction in E was recorded for PrD. Higher quantities of ICTP and CTX were released from PrD after 1 week. Conclusions: Primary and permanent teeth presented similar demineralization rates. Collagen degradation, however, was faster and more substantial for PrD.

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Proteolytic activity, degradation, and dissolution of primary and permanent teeth

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ASJC Scopus subject areas

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