Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh; Jeffrey J. Campion; Belal Y. Gharaibeh; Martin E. Evans; Kozo Saito

doi:10.1016/j.infrared.2011.11.004

Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Ahmad A. Salaimeh
, Jeffrey J. Campion
, Belal Y. Gharaibeh
, Martin E. Evans
, Kozo Saito

Institute of Research for Technology Development (IR4TD)

Producción científica: Article › revisión exhaustiva

10 Citas (Scopus)

Resumen

We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 ⁸ CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

Idioma original	English
Páginas (desde-hasta)	170-172
Número de páginas	3
Publicación	Infrared Physics and Technology
Volumen	55
N.º	1
DOI	https://doi.org/10.1016/j.infrared.2011.11.004
Estado	Published - ene 2012

Nota bibliográfica

Funding Information:
We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

Financiación

We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

Financiadores	Número del financiador
University of Kentucky Institute of Research for Technology Development	IR4TD
University of Kentucky

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics

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10.1016/j.infrared.2011.11.004

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title = "Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography",

abstract = "We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 {\texttimes} 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.",

keywords = "Bacterial quantification, Energy content, Infrared thermography, Real-time, Staphylococcus aureus",

author = "Salaimeh, \{Ahmad A.\} and Campion, \{Jeffrey J.\} and Gharaibeh, \{Belal Y.\} and Evans, \{Martin E.\} and Kozo Saito",

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AU - Campion, Jeffrey J.

AU - Gharaibeh, Belal Y.

AU - Evans, Martin E.

AU - Saito, Kozo

N1 - Funding Information: We would like to thank Judy Glass for help with the experiments, and Mary Lou Cahal for assistance in preparing the figures. This study was supported in part by a fundamental research grant from the Institute of Research for Technology Development (IR4TD), University of Kentucky and with use of laboratory facilities at the Lexington Veterans Affairs Medical Center, Lexington, Kentucky.

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N2 - We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

AB - We previously showed that infrared thermography (IRT) could be used to quantify viable Escherichia coli, a representative gram-negative bacterium, in liquid growth media. Here, we evaluated the ability of IRT to enumerate a viable representative gram-positive organism, Staphylococcus aureus. We found that the energy content (EC) of the media was strongly positively correlated (r = 0.999) to measured viable counts of S. aureus ranging from 85 colony-forming units (CFU)/ml to ∼4 × 10 8 CFU/ml. The EC of S. aureus was ∼2-fold higher than that of E. coli at comparable cell concentrations suggesting that IRT may be used to distinguish genera.

KW - Bacterial quantification

KW - Energy content

KW - Infrared thermography

KW - Real-time

KW - Staphylococcus aureus

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DO - 10.1016/j.infrared.2011.11.004

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SP - 170

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JO - Infrared Physics and Technology

JF - Infrared Physics and Technology

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Real-time quantification of Staphylococcus aureus in liquid medium using infrared thermography

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

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