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Response of neuroglia to hypoxia-induced oxidative stress using enzymatically crosslinked hydrogels

  • Samantha G. Zambuto
  • , Julio F. Serrano
  • , Avery C. Vilbert
  • , Yi Lu
  • , Brendan A.C. Harley
  • , Sara Pedron

Producción científica: Articlerevisión exhaustiva

18 Citas (Scopus)

Resumen

Three-dimensional cultures have exciting potential to mimic aspects of healthy and diseased brain tissue to examine the role of physiological conditions on neural biomarkers, as well as disease onset and progression. Hypoxia is associated with oxidative stress, mitochondrial damage, and inflammation, key processes potentially involved in Alzheimer's and multiple sclerosis. We describe the use of an enzymatically-crosslinkable gelatin hydrogel system within a microfluidic device to explore the effects of hypoxia-induced oxidative stress on rat neuroglia, human astrocyte reactivity, and myelin production. This versatile platform offers new possibilities for drug discovery and modeling disease progression.

Idioma originalEnglish
Páginas (desde-hasta)83-90
Número de páginas8
PublicaciónMRS Communications
Volumen10
N.º1
DOI
EstadoPublished - mar 1 2020

Nota bibliográfica

Publisher Copyright:
© Copyright Materials Research Society 2019.

Financiación

The authors acknowledge Dr. Austin Cyphersmith (IGB, University of Illinois) for the assistance with fluorescence imaging, Mai Ngo for the assistance with microfluidic chips, and Zona Hrnjak and Aidan Gilchrist for the compression testing methodology and the custom MATLAB code for modulus analysis. The authors gratefully acknowledge support from the Scott H. Fisher IGB Graduate Student Research Fund. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health (NIH) under Award Number R01 CA197488, the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number R01 DK099528, as well as the Chemical Transformations Initiative at Pacific Northwest National Laboratory (PNNL) under the Laboratory Directed Research and Development Program at PNNL, a multiprogram national laboratory operated by Battelle for the U.S. Department of Energy (DOE). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or the DOE. The authors are also grateful for additional funding provided by the Department of Chemical & Biomolecular Engineering and the Carl R. Woese Institute for Genomic Biology at the University of Illinois at Urbana-Champaign.

FinanciadoresNúmero del financiador
Scott H. Fisher IGB Graduate Student Research Fund
National Institutes of Health (NIH)R01 CA197488
U.S. Department of Energy EPSCoR
National Childhood Cancer Registry – National Cancer Institute
National Institute of Diabetes and Digestive and Kidney DiseasesR01 DK099528
Laboratory Directed Research and Development
Pacific Northwest National Laboratory

    ODS de las Naciones Unidas

    Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

    1. Good health and well being
      Good health and well being

    ASJC Scopus subject areas

    • General Materials Science

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