S100β expression in Alzheimer's disease: Relation to neuropathology in brain regions

Linda J. Van Eldik; W. Sue T. Griffin

doi:10.1016/0167-4889(94)90101-5

S100β expression in Alzheimer's disease: Relation to neuropathology in brain regions

Linda J. Van Eldik
, W. Sue T. Griffin

Producción científica: Article › revisión exhaustiva

196 Citas (Scopus)

Resumen

S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.

Idioma original	English
Páginas (desde-hasta)	398-403
Número de páginas	6
Publicación	Biochimica et Biophysica Acta - Molecular Cell Research
Volumen	1223
N.º	3
DOI	https://doi.org/10.1016/0167-4889(94)90101-5
Estado	Published - sept 29 1994

Nota bibliográfica

Funding Information:
These studies were supported in part by NIH grant AG10208 (to L.V.E. and W.S.T.G.). We especially thank Dr. Jin G. Sheng for technical assistance and Dr. Robert E. Mrak for review and comment on the manuscript and Anna Feoktistova, Cynthia Rovnaghi, and Dr. Olcay Jones for assistance in various aspects of this work.

Financiación

These studies were supported in part by NIH grant AG10208 (to L.V.E. and W.S.T.G.). We especially thank Dr. Jin G. Sheng for technical assistance and Dr. Robert E. Mrak for review and comment on the manuscript and Anna Feoktistova, Cynthia Rovnaghi, and Dr. Olcay Jones for assistance in various aspects of this work.

Financiadores	Número del financiador
National Institutes of Health (NIH)
National Institute on Aging	P01AG010208

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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@article{02e55b7f695f4af2b570ecce111423cc,

title = "S100β expression in Alzheimer's disease: Relation to neuropathology in brain regions",

abstract = "S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.",

keywords = "Alzheimer's disease, Cytokine, ELISA, Glial activation, Immunohistochemistry, S100β",

author = "\{Van Eldik\}, \{Linda J.\} and Griffin, \{W. Sue T.\}",

note = "Funding Information: These studies were supported in part by NIH grant AG10208 (to L.V.E. and W.S.T.G.). We especially thank Dr. Jin G. Sheng for technical assistance and Dr. Robert E. Mrak for review and comment on the manuscript and Anna Feoktistova, Cynthia Rovnaghi, and Dr. Olcay Jones for assistance in various aspects of this work.",

year = "1994",

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doi = "10.1016/0167-4889(94)90101-5",

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AU - Van Eldik, Linda J.

AU - Griffin, W. Sue T.

N1 - Funding Information: These studies were supported in part by NIH grant AG10208 (to L.V.E. and W.S.T.G.). We especially thank Dr. Jin G. Sheng for technical assistance and Dr. Robert E. Mrak for review and comment on the manuscript and Anna Feoktistova, Cynthia Rovnaghi, and Dr. Olcay Jones for assistance in various aspects of this work.

PY - 1994/9/29

Y1 - 1994/9/29

N2 - S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.

AB - S100β levels in tissue homogenates and distribution of S100β-containing activated astrocytes were examined by S100β-specific ELISA and immunohistochemistry, respectively, in brain regions exhibiting many, some, few, or no neuritic plaques in Alzheimer's disease (AD). Compared to samples collected at similar postmortem intervals from control patients of similar ages, S100β levels were elevated in specific brain regions from AD patients, and the overexpression of S100β correlated relatively well with the pattern of regional involvement by neuritic plaques. The largest increases in S100β levels were in hippocampus and temporal lobe, followed by frontal lobe and pons, with no elevation in occipital lobe or cerebellum. Immunohistochemical analysis showed S100β localization primarily in activated astrocytes surrounding neuritic plaques. These results demonstrate that S100β overexpression is brain region-specific and related to astrocyte activation and suggest that elevation of S100β above some threshold is related to the degree of neuropathological involvement of different brain regions in AD.

KW - Alzheimer's disease

KW - Cytokine

KW - ELISA

KW - Glial activation

KW - Immunohistochemistry

KW - S100β

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M3 - Article

C2 - 7918676

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S100β expression in Alzheimer's disease: Relation to neuropathology in brain regions

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ASJC Scopus subject areas

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