Screening a fragment cocktail library using ultrafiltration

Sayaka Shibata; Zhongsheng Zhang; Konstantin V. Korotkov; Jaclyn Delarosa; Alberto Napuli; Angela M. Kelley; Natasha Mueller; Jennifer Ross; Frank H. Zucker; Frederick S. Buckner; Ethan A. Merritt; Christophe L.M.J. Verlinde; Wesley C. Van Voorhis; Wim G.J. Hol; Erkang Fan

doi:10.1007/s00216-011-5225-7

Screening a fragment cocktail library using ultrafiltration

Sayaka Shibata
, Zhongsheng Zhang
, Konstantin V. Korotkov
, Jaclyn Delarosa
, Alberto Napuli
, Angela M. Kelley
, Natasha Mueller
, Jennifer Ross
, Frank H. Zucker
, Frederick S. Buckner
, Ethan A. Merritt
, Christophe L.M.J. Verlinde
, Wesley C. Van Voorhis
, Wim G.J. Hol
, Erkang Fan

Producción científica: Article › revisión exhaustiva

9 Citas (Scopus)

Resumen

Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K _d, the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-sized molecules, with molecular masses between 150 and 300 Da, were screened against medical structural genomics target proteins. The validity of the method was confirmed through competitive binding assays in the presence of ligands known to bind the target proteins.

Idioma original	English
Páginas (desde-hasta)	1589-1595
Número de páginas	7
Publicación	Analytical and Bioanalytical Chemistry
Volumen	401
N.º	5
DOI	https://doi.org/10.1007/s00216-011-5225-7
Estado	Published - sept 2011

Nota bibliográfica

Funding Information:
We thank Eric T. Larson for his critical review of the manuscript. This work was supported by National Institutes of Health grants P50GM64655 (SGPP), P01AI067921 (MSGPP), and AI34501.

Financiación

We thank Eric T. Larson for his critical review of the manuscript. This work was supported by National Institutes of Health grants P50GM64655 (SGPP), P01AI067921 (MSGPP), and AI34501.

Financiadores	Número del financiador
MSGPP
National Institutes of Health (NIH)	P50GM64655, P01AI067921
National Institute of Allergy and Infectious Diseases	R56AI034501

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1007/s00216-011-5225-7

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Shibata, S., Zhang, Z., Korotkov, K. V., Delarosa, J., Napuli, A., Kelley, A. M., Mueller, N., Ross, J., Zucker, F. H., Buckner, F. S., Merritt, E. A., Verlinde, C. L. M. J., Van Voorhis, W. C., Hol, W. G. J., & Fan, E. (2011). Screening a fragment cocktail library using ultrafiltration. Analytical and Bioanalytical Chemistry, 401(5), 1589-1595. https://doi.org/10.1007/s00216-011-5225-7

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title = "Screening a fragment cocktail library using ultrafiltration",

abstract = "Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K d, the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-sized molecules, with molecular masses between 150 and 300 Da, were screened against medical structural genomics target proteins. The validity of the method was confirmed through competitive binding assays in the presence of ligands known to bind the target proteins.",

keywords = "Compound library, Fragment-based drug discovery, Screening, Ultrafiltration",

author = "Sayaka Shibata and Zhongsheng Zhang and Korotkov, \{Konstantin V.\} and Jaclyn Delarosa and Alberto Napuli and Kelley, \{Angela M.\} and Natasha Mueller and Jennifer Ross and Zucker, \{Frank H.\} and Buckner, \{Frederick S.\} and Merritt, \{Ethan A.\} and Verlinde, \{Christophe L.M.J.\} and \{Van Voorhis\}, \{Wesley C.\} and Hol, \{Wim G.J.\} and Erkang Fan",

note = "Funding Information: We thank Eric T. Larson for his critical review of the manuscript. This work was supported by National Institutes of Health grants P50GM64655 (SGPP), P01AI067921 (MSGPP), and AI34501.",

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doi = "10.1007/s00216-011-5225-7",

language = "English",

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AU - Shibata, Sayaka

AU - Zhang, Zhongsheng

AU - Korotkov, Konstantin V.

AU - Delarosa, Jaclyn

AU - Napuli, Alberto

AU - Kelley, Angela M.

AU - Mueller, Natasha

AU - Ross, Jennifer

AU - Zucker, Frank H.

AU - Buckner, Frederick S.

AU - Merritt, Ethan A.

AU - Verlinde, Christophe L.M.J.

AU - Van Voorhis, Wesley C.

AU - Hol, Wim G.J.

AU - Fan, Erkang

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N2 - Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K d, the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-sized molecules, with molecular masses between 150 and 300 Da, were screened against medical structural genomics target proteins. The validity of the method was confirmed through competitive binding assays in the presence of ligands known to bind the target proteins.

AB - Ultrafiltration provides a generic method to discover ligands for protein drug targets with millimolar to micromolar K d, the typical range of fragment-based drug discovery. This method was tailored to a 96-well format, and cocktails of fragment-sized molecules, with molecular masses between 150 and 300 Da, were screened against medical structural genomics target proteins. The validity of the method was confirmed through competitive binding assays in the presence of ligands known to bind the target proteins.

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KW - Fragment-based drug discovery

KW - Screening

KW - Ultrafiltration

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Screening a fragment cocktail library using ultrafiltration

Resumen

Nota bibliográfica

Financiación

ASJC Scopus subject areas

Acceder al documento

Otros archivos y enlaces

Huella

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