Specific immunological detection of the (V+C)- fibronectin isoform

Nancy Burton-Wurster; Hao Chen; Rina Gendelman; Michael L. Jackson; Lucille F. Gagliardo; Da Nian Gu; Russell R. Zelko; George Lust; James N. MacLeod

doi:10.1016/S0945-053X(02)00033-1

Specific immunological detection of the (V+C)^- fibronectin isoform

Nancy Burton-Wurster
, Hao Chen
, Rina Gendelman
, Michael L. Jackson
, Lucille F. Gagliardo
, Da Nian Gu
, Russell R. Zelko
, George Lust
, James N. MacLeod

Producción científica: Article › revisión exhaustiva

2 Citas (Scopus)

Resumen

The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)^- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)^- fibronectin without cross-recognition of V⁺C⁺ and V^-C⁺ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)^-, displays specificity for (V+C)^- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

Idioma original	English
Páginas (desde-hasta)	393-398
Número de páginas	6
Publicación	Matrix Biology
Volumen	21
N.º	5
DOI	https://doi.org/10.1016/S0945-053X(02)00033-1
Estado	Published - ago 2002

Nota bibliográfica

Funding Information:
This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

Financiación

This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

Financiadores	Número del financiador
National Institutes of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases	R01AR044340
Arthritis Foundation

ASJC Scopus subject areas

Molecular Biology

Acceder al documento

10.1016/S0945-053X(02)00033-1

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title = "Specific immunological detection of the (V+C)- fibronectin isoform",

abstract = "The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.",

keywords = "(V+C) fibronectin, Biomarker, Cartilage, Monoclonal antibody, Osteoarthritis, Synovial fluid",

author = "Nancy Burton-Wurster and Hao Chen and Rina Gendelman and Jackson, \{Michael L.\} and Gagliardo, \{Lucille F.\} and Gu, \{Da Nian\} and Zelko, \{Russell R.\} and George Lust and MacLeod, \{James N.\}",

note = "Funding Information: This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.",

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AU - Chen, Hao

AU - Gendelman, Rina

AU - Jackson, Michael L.

AU - Gagliardo, Lucille F.

AU - Gu, Da Nian

AU - Zelko, Russell R.

AU - Lust, George

AU - MacLeod, James N.

N1 - Funding Information: This work was supported by NIH grant AR44340 and the Arthritis Foundation. We acknowledge the invaluable assistance of Dr Judith Appleton in the production of the monoclonal antibody. We thank Ms Bevin Zimmerman and Ms Alina Jo Williams for excellent technical help. We are grateful to Dr Jean Schwarzbauer of Princeton University for gifts of FN1, DN1 and DN2 from which the FN3, DN3, and all other expression constructs were derived, and for Mab IC3 that is specific for all isoforms of rat fibronectin. The fibronectin null mouse embryo fibroblasts were generous gifts from Dr Dean Mosher at the University of Wisconsin, Madison. We appreciate the invaluable secretarial support of Ms Dorothy Scorelle.

PY - 2002/8

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N2 - The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

AB - The population of fibronectins in adult mammalian cartilage includes high levels of a cartilage-specific (V+C)- isoform which lacks the V, III-15, and I-10 segments and thus contains a novel junction between protein segments III-14 and I-11. We report production of a monoclonal antibody specific for (V+C)- fibronectin without cross-recognition of V+C+ and V-C+ isoforms found in plasma and other tissues. Presentation of epitope to this antibody requires the III-14/I-11 junction, but the epitope itself extends beyond 14 amino acids immediately surrounding the junction site and involves a conformational change in III-14 and/or the N-terminal portion of I-11. The antibody, designated Mab 5D10 anti (V+C)-, displays specificity for (V+C)- fibronectin from multiple mammalian species including humans and utility in immunoblots, immunohistochemistry, and ELISA.

KW - (V+C) fibronectin

KW - Biomarker

KW - Cartilage

KW - Monoclonal antibody

KW - Osteoarthritis

KW - Synovial fluid

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