Standardisation of the quantitation of serum amyloid A protein (SAA) in human serum

An adequate method for standardising the quantitation of serum amyloid A protein (SSA) in human serum was developed. Acute phase high density lipoprotien₃ (HDL₃) was used as a standard. The concentration of the SAA in the standard was determined by the use of purified SAA. After protein determination, various concentrations of purified SAA were run on SDS-polyacylamide gel together with the HDL₃ standard containing an unknown amount of SAA amongst the apolipoproteins. From the standard curve obtained by pyridine extraction (Coomassie blue colour yield at A₆₀₅ nm) the concentration of SAA in the HDL₃ standard was determined. An established immunoradiometric assay (IRMA) for SAA was standardised with the HDL₃. SAA concentration in normal and acute phase sera were determined.