TY - GEN
T1 - Standardized median difference for quality control in high-throughput screening
AU - Huang, Wenxian
AU - Zhang, Xiaohua Douglas
AU - Li, Yong
AU - Wang, William Wubao
AU - Soper, Keith
PY - 2012
Y1 - 2012
N2 - High-throughput screening (HTS) of large-scale ribonucleic acid interference (RNAi) libraries has been widely used in functional genomics in recent years. The Z-factor and strictly standardized mean difference (SSMD) have already become two popular methods for quality control (QC) in HTS experiments. This paper is intended to put forward a robust parameter, standardized median difference (SMD), for group comparison and derive some statistical properties of SMD, such as maximum likelihood estimate (MLE), moment-method (MM) estimate and plug-in estimate of SMD as well as their relative asymptotical distributions. Using the simulations, the plug-in estimate of SMD obtains QC results robust to outliers whereas the Z-factor and SSMD obtain QC results sensitive to outliers. Meanwhile, compared to other QC metrics, the SMD also obtains non-inferior QC results when there are no outliers.
AB - High-throughput screening (HTS) of large-scale ribonucleic acid interference (RNAi) libraries has been widely used in functional genomics in recent years. The Z-factor and strictly standardized mean difference (SSMD) have already become two popular methods for quality control (QC) in HTS experiments. This paper is intended to put forward a robust parameter, standardized median difference (SMD), for group comparison and derive some statistical properties of SMD, such as maximum likelihood estimate (MLE), moment-method (MM) estimate and plug-in estimate of SMD as well as their relative asymptotical distributions. Using the simulations, the plug-in estimate of SMD obtains QC results robust to outliers whereas the Z-factor and SSMD obtain QC results sensitive to outliers. Meanwhile, compared to other QC metrics, the SMD also obtains non-inferior QC results when there are no outliers.
UR - http://www.scopus.com/inward/record.url?scp=84867713019&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867713019&partnerID=8YFLogxK
U2 - 10.1109/ITiME.2012.6291354
DO - 10.1109/ITiME.2012.6291354
M3 - Conference contribution
AN - SCOPUS:84867713019
SN - 9781467321068
T3 - Proceedings of 2012 International Symposium on Information Technologies in Medicine and Education, ITME 2012
SP - 515
EP - 518
BT - Proceedings of 2012 International Symposium on Information Technologies in Medicine and Education, ITME 2012
T2 - 2012 International Symposium on Information Technologies in Medicine and Education, ITME 2012
Y2 - 3 August 2012 through 5 August 2012
ER -