Strong HCV NS3- and NS4A-specific cellular immune responses induced in mice and Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine

Krystle A. Lang; Jian Yan; Ruxandra Draghia-Akli; Amir Khan; David B. Weiner

doi:10.1016/j.vaccine.2008.07.052

Strong HCV NS3- and NS4A-specific cellular immune responses induced in mice and Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine

Krystle A. Lang
, Jian Yan
, Ruxandra Draghia-Akli
, Amir Khan
, David B. Weiner

Producción científica: Article › revisión exhaustiva

37 Citas (Scopus)

Resumen

Hepatitis C virus (HCV) represents a major health burden with more than 170 million individuals currently infected worldwide, equaling roughly 3% of the world's population. HCV preferentially infects hepatocytes and is able to persist in up to 70% of infected individuals. It is estimated that up to 30% of chronically infected individuals will go on to develop progressive liver disease as a result of HCV infection, making the virus the leading cause of liver transplantation in the world. Currently there is no vaccine for HCV. In this study, we have taken a multi-step approach to develop a novel genotype 1a/1b consensus HCV NS3/NS4A DNA vaccine able to induce strong cellular immunity. We show that this construct is able to induce strong anti-NS3/NS4A T cell responses in C57BL/6 mice, as well as, in Rhesus macaques. Our data suggest that DNA vaccines encoding HCV proteins NS3/NS4A merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.

Idioma original	English
Páginas (desde-hasta)	6225-6231
Número de páginas	7
Publicación	Vaccine
Volumen	26
N.º	49
DOI	https://doi.org/10.1016/j.vaccine.2008.07.052
Estado	Published - nov 18 2008

Nota bibliográfica

Funding Information:
This manuscript was supported in part by funding from the NIH to DBW and from VGX Pharmaceuticals, Inc. The laboratory notes possible commercial conflicts associated with this work, which may include; Wyeth, VGX, BMS, Virxsys, Ichor, Merck, Althea, & Aldeveron.

Financiación

This manuscript was supported in part by funding from the NIH to DBW and from VGX Pharmaceuticals, Inc. The laboratory notes possible commercial conflicts associated with this work, which may include; Wyeth, VGX, BMS, Virxsys, Ichor, Merck, Althea, & Aldeveron.

Financiadores	Número del financiador
VGX Pharmaceuticals, Inc.
National Institutes of Health (NIH)
National Institute of Allergy and Infectious Diseases	P01AI071739

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Good health and well being

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2008.07.052

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title = "Strong HCV NS3- and NS4A-specific cellular immune responses induced in mice and Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine",

abstract = "Hepatitis C virus (HCV) represents a major health burden with more than 170 million individuals currently infected worldwide, equaling roughly 3\% of the world's population. HCV preferentially infects hepatocytes and is able to persist in up to 70\% of infected individuals. It is estimated that up to 30\% of chronically infected individuals will go on to develop progressive liver disease as a result of HCV infection, making the virus the leading cause of liver transplantation in the world. Currently there is no vaccine for HCV. In this study, we have taken a multi-step approach to develop a novel genotype 1a/1b consensus HCV NS3/NS4A DNA vaccine able to induce strong cellular immunity. We show that this construct is able to induce strong anti-NS3/NS4A T cell responses in C57BL/6 mice, as well as, in Rhesus macaques. Our data suggest that DNA vaccines encoding HCV proteins NS3/NS4A merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.",

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note = "Funding Information: This manuscript was supported in part by funding from the NIH to DBW and from VGX Pharmaceuticals, Inc. The laboratory notes possible commercial conflicts associated with this work, which may include; Wyeth, VGX, BMS, Virxsys, Ichor, Merck, Althea, \& Aldeveron.",

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doi = "10.1016/j.vaccine.2008.07.052",

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AU - Weiner, David B.

N1 - Funding Information: This manuscript was supported in part by funding from the NIH to DBW and from VGX Pharmaceuticals, Inc. The laboratory notes possible commercial conflicts associated with this work, which may include; Wyeth, VGX, BMS, Virxsys, Ichor, Merck, Althea, & Aldeveron.

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N2 - Hepatitis C virus (HCV) represents a major health burden with more than 170 million individuals currently infected worldwide, equaling roughly 3% of the world's population. HCV preferentially infects hepatocytes and is able to persist in up to 70% of infected individuals. It is estimated that up to 30% of chronically infected individuals will go on to develop progressive liver disease as a result of HCV infection, making the virus the leading cause of liver transplantation in the world. Currently there is no vaccine for HCV. In this study, we have taken a multi-step approach to develop a novel genotype 1a/1b consensus HCV NS3/NS4A DNA vaccine able to induce strong cellular immunity. We show that this construct is able to induce strong anti-NS3/NS4A T cell responses in C57BL/6 mice, as well as, in Rhesus macaques. Our data suggest that DNA vaccines encoding HCV proteins NS3/NS4A merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.

AB - Hepatitis C virus (HCV) represents a major health burden with more than 170 million individuals currently infected worldwide, equaling roughly 3% of the world's population. HCV preferentially infects hepatocytes and is able to persist in up to 70% of infected individuals. It is estimated that up to 30% of chronically infected individuals will go on to develop progressive liver disease as a result of HCV infection, making the virus the leading cause of liver transplantation in the world. Currently there is no vaccine for HCV. In this study, we have taken a multi-step approach to develop a novel genotype 1a/1b consensus HCV NS3/NS4A DNA vaccine able to induce strong cellular immunity. We show that this construct is able to induce strong anti-NS3/NS4A T cell responses in C57BL/6 mice, as well as, in Rhesus macaques. Our data suggest that DNA vaccines encoding HCV proteins NS3/NS4A merit further study in the context of future prophylactic and therapeutic HCV T cell based vaccines.

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KW - HCV

KW - NS3

KW - NS4A

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Strong HCV NS3- and NS4A-specific cellular immune responses induced in mice and Rhesus macaques by a novel HCV genotype 1a/1b consensus DNA vaccine

Resumen

Nota bibliográfica

Financiación

ODS de las Naciones Unidas

ASJC Scopus subject areas

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