TY - JOUR
T1 - Structural characterization of CalO1
T2 - A putative orsellinic acid methyltransferase in the calicheamicin-biosynthetic pathway
AU - Chang, Aram
AU - Singh, Shanteri
AU - Bingman, Craig A.
AU - Thorson, Jon S.
AU - Phillips, George N.
PY - 2011/3
Y1 - 2011/3
N2 - The X-ray structure determination at 2.4 Å resolution of the putative orsellinic acid C3 O-methyltransferase (CalO1) involved in calicheamicin biosynthesis is reported. Comparison of CalO1 with a homology model of the functionally related calicheamicin orsellinic acid C2 O-methyltransferase (CalO6) implicates several residues that are likely to contribute to the regiospecificity of alkylation. Consistent with the proposed requirement of an acyl-carrier-protein-bound substrate, this structural study also reveals structural determinants within CalO1 that are anticipated to accommodate an association with an acyl carrier protein.
AB - The X-ray structure determination at 2.4 Å resolution of the putative orsellinic acid C3 O-methyltransferase (CalO1) involved in calicheamicin biosynthesis is reported. Comparison of CalO1 with a homology model of the functionally related calicheamicin orsellinic acid C2 O-methyltransferase (CalO6) implicates several residues that are likely to contribute to the regiospecificity of alkylation. Consistent with the proposed requirement of an acyl-carrier-protein-bound substrate, this structural study also reveals structural determinants within CalO1 that are anticipated to accommodate an association with an acyl carrier protein.
KW - S-adenosylmethionine
KW - acyl carrier proteins
KW - biosynthesis
KW - enediynes
KW - natural products
KW - polyketides
UR - https://www.scopus.com/pages/publications/79952269133
UR - https://www.scopus.com/inward/citedby.url?scp=79952269133&partnerID=8YFLogxK
U2 - 10.1107/S090744491100360X
DO - 10.1107/S090744491100360X
M3 - Article
C2 - 21358050
AN - SCOPUS:79952269133
SN - 0907-4449
VL - 67
SP - 197
EP - 203
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 3
ER -